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Table 3 Two hour in vitro cortisol responses to hormonal stimuli in primary adrenal cell cultures

From: ACTH-independent macronodular adrenocortical hyperplasia reveals prevalent aberrant in vivo and in vitroresponses to hormonal stimuli and coupling of arginine-vasopressin type 1a receptor to 11β-hydroxylase

  

AIMAH

 

Non-AIMAH

 

Dose

n

Partial response (%)

Full response (%)

Correlation with in vivotest

n

Partial response (%)

Full response (%)

ACTH1-24

50 pg/ml

7

2 (29%)

4 (57%)

0.30

5

1 (20%)

4 (80%)

ACTH1-24

500 pg/ml

10

3 (30%)

6 (60%)

0.07

18

1 (6%)

16 (89%)

FSK

1 μM

8

0

7 (88%)

0.29

9

0

7 (78%)

AngII

10 nM

2

0

0

n.a. #

2

1 (50%)

1 (50%)

AVP

10 nM

6

2 (33%)

1 (17%)

−0.72; -0.50#

4

1 (25%)

0

AVP

100 nM

4

1 (25%)

1 (25%)

−1.00; -0.80#

3

1 (33%)

0

Desmopressin

10 nM

5

0

0

−0.80

3

0

0

Metoclopramide

1 μM

7

0

2 (29%)

0.71

14

3 (21%)

0

Epinephrine

1 μM

7

1 (14%)

1 (14%)

−0.50#

5

0

0

Norepinephrine

1 μM

7

1 (14%)

0

−0.15#

5

0

0

hCG

100 IU/ml

10

3 (30%)

0

0.60

4

0

0

TSH

10 nM

4

0

0

−0.32

1

0

0

Glucagon

1 μM

4

0

0

0.50

1

0

0

GIP

100 nM

6

1 (17%)

1 (17%)

0.49

4

1 (25%)

0

  1. standard mixed meal, # upright posture test, AVP im, n.a.: not applicable.