ACTH-independent macronodular adrenocortical hyperplasia reveals prevalent aberrant in vivo and in vitroresponses to hormonal stimuli and coupling of arginine-vasopressin type 1a receptor to 11β-hydroxylase

Table 3 Two hour in vitro cortisol responses to hormonal stimuli in primary adrenal cell cultures

		AIMAH				Non-AIMAH
	Dose	n	Partial response (%)	Full response (%)	Correlation with *in vivo*test	n	Partial response (%)	Full response (%)
ACTH_1-24	50 pg/ml	7	2 (29%)	4 (57%)	0.30	5	1 (20%)	4 (80%)
ACTH_1-24	500 pg/ml	10	3 (30%)	6 (60%)	0.07	18	1 (6%)	16 (89%)
FSK	1 μM	8	0	7 (88%)	0.29	9	0	7 (78%)
AngII	10 nM	2	0	0	n.a. ^#	2	1 (50%)	1 (50%)
AVP	10 nM	6	2 (33%)	1 (17%)	−0.72^†; -0.50^#	4	1 (25%)	0
AVP	100 nM	4	1 (25%)	1 (25%)	−1.00^†; -0.80^#	3	1 (33%)	0
Desmopressin	10 nM	5	0	0	−0.80	3	0	0
Metoclopramide	1 μM	7	0	2 (29%)	0.71	14	3 (21%)	0
Epinephrine	1 μM	7	1 (14%)	1 (14%)	−0.50^#	5	0	0
Norepinephrine	1 μM	7	1 (14%)	0	−0.15^#	5	0	0
hCG	100 IU/ml	10	3 (30%)	0	0.60	4	0	0
TSH	10 nM	4	0	0	−0.32	1	0	0
Glucagon	1 μM	4	0	0	0.50	1	0	0
GIP	100 nM	6	1 (17%)	1 (17%)	0.49^¶	4	1 (25%)	0

^¶ standard mixed meal, ^# upright posture test, ^† AVP im, n.a.: not applicable.

ISSN: 1750-1172