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Table 3 Two hour in vitro cortisol responses to hormonal stimuli in primary adrenal cell cultures

From: ACTH-independent macronodular adrenocortical hyperplasia reveals prevalent aberrant in vivo and in vitroresponses to hormonal stimuli and coupling of arginine-vasopressin type 1a receptor to 11β-hydroxylase

   AIMAH   Non-AIMAH
  Dose n Partial response (%) Full response (%) Correlation with in vivotest n Partial response (%) Full response (%)
ACTH1-24 50 pg/ml 7 2 (29%) 4 (57%) 0.30 5 1 (20%) 4 (80%)
ACTH1-24 500 pg/ml 10 3 (30%) 6 (60%) 0.07 18 1 (6%) 16 (89%)
FSK 1 μM 8 0 7 (88%) 0.29 9 0 7 (78%)
AngII 10 nM 2 0 0 n.a. # 2 1 (50%) 1 (50%)
AVP 10 nM 6 2 (33%) 1 (17%) −0.72; -0.50# 4 1 (25%) 0
AVP 100 nM 4 1 (25%) 1 (25%) −1.00; -0.80# 3 1 (33%) 0
Desmopressin 10 nM 5 0 0 −0.80 3 0 0
Metoclopramide 1 μM 7 0 2 (29%) 0.71 14 3 (21%) 0
Epinephrine 1 μM 7 1 (14%) 1 (14%) −0.50# 5 0 0
Norepinephrine 1 μM 7 1 (14%) 0 −0.15# 5 0 0
hCG 100 IU/ml 10 3 (30%) 0 0.60 4 0 0
TSH 10 nM 4 0 0 −0.32 1 0 0
Glucagon 1 μM 4 0 0 0.50 1 0 0
GIP 100 nM 6 1 (17%) 1 (17%) 0.49 4 1 (25%) 0
  1. standard mixed meal, # upright posture test, AVP im, n.a.: not applicable.