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Figure 2 | Orphanet Journal of Rare Diseases

Figure 2

From: Compounds that correct F508del-CFTR trafficking can also correct other protein trafficking diseases: an in vitro study using cell lines

Figure 2

F508del-CFTR trafficking correctors have mixed effects on the trafficking of wild type hERG. (A) Representative blot showing wt hERG treated with selected trafficking correctors as labeled above the immunoblot. Arrows indicate core and complex-glycosylated hERG. Tubulin serves as a loading control. (B) siRNA knockdown of PDE5A does not correct the trafficking of hERG G601S. Top : hERG G601S cells treated with vehicle (DMSO), 1 μM astemizole as a positive control, siRNA transfection reagent alone (HiPerfect), non-targeting siRNA, or two separate wells transfected with 25 nM siRNA against PDE5A. Arrows indicate core and complex-glycosylated hERG. Middle : PDE5A expression levels in each of the samples described. Bottom : Tubulin serves as a loading control.

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172