Table 1 RT-PCR assay details: PCR primer and probe sequences, or TaqMan™ assay ID
Assay specification | Assay ID | Assay details |
|---|---|---|
Detects Normal MeCP2_E1 transcript only | MeCP2_E1 NL | Left 5′-CGGAGGAGGAGGAGGA-3′ |
Right 5′-GGAGGTCCTGGTCTTCTGACTT-3′ | ||
Probe 6FAM-5′-AGGAGGAGAGACTGGAA3′ | ||
Detects Mutated MeCP2_E1 transcript only | MeCP2_E1 Mut | Left 5′-GTAAAAGCCGTCCGGAAAAT-3′ |
Right 5′-TGCTTGCCCTCTTTCTCTTC-3′ | ||
Probe 6FAM-5′-AGGAGGAGGGAAGAAA-3′ | ||
Detects MeCP2_E2 transcript only | MeCP2_E2 | TaqMan™ Pre-Designed Gene expression Assay |
ID number: Hs00172845 | ||
Detects PGK1 transcript only (Endogenous control) | PGK1 | TaqMan™ Pre-Designed Gene expression Assay |
ID number: Hs99999906 |