Figure 6
From: Rescue of nonsense mutations by amlexanox in human cells
Amlexanox treatment of 6CFSMEo- cells leads to the synthesis of functional CFTR. 6CFSMEo- cells were loaded with the halide-sensitive fluorophore SPQ and treated with DMSO (■), 0.2 μM amlexanox (Δ), 1 μM amlexanox (●), 5 μM amlexanox (□) or 25 μM amlexanox (▴) for 20 hours. At time (t) = 2 min cAMP-stimulating cocktail was added (arrow). The increase in iodide efflux is shown as mean ± SEM from at least four independent experiments. Ft represents the measure of the fluorescence at the reading time; F0 is an average fluorescence before addition of cAMP agonists.