Figure 5
From: Rescue of nonsense mutations by amlexanox in human cells
Dystrophin is found at the cell membrane of DMD cells after amlexanox treatment. WT (left panel) or DMD cells (two right panels) were incubated in differentiation medium in presence of DMSO or 5 μM of amlexanox for 48 hours. Then cells were fixed and nuclei (blue) were stained using Hoechst reagent and dystrophin (green) was localized using anti-dystrophin raised against the C-terminal part of the protein. White arrows indicate dystrophin localization at the cellular membrane. Scale bar represents 10 μm.