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Table 4 Incongruent results obtained by the BIOCHIP mosaic and the conventional procedure

From: Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

Conventional procedure Biochip mosaic
Diagnose Findings Diagnose Findings
LAD, n = 6 2 with linear IgA deposits on salt-split skin, 4 with anti-LAD-1 IgA reactivity NS, n = 5; pemphigoid, n = 1 1 with linear IgG/IgA deposits on salt-split skin; 5 without IR
p200, n = 5 linear IgG deposits on salt-split skin and anti-p200 protein/ laminin γ1 reactivity NS, n = 3; pemphigoid, n = 2 2 with linear IgG/IgA deposits on monkey esophagus and salt split skin; 3 without IR
MMP, n = 6 3 with anti-LAD-1 IgG reactivity, 3 with anti-laminin 332 reactivity NS, n = 4; pemphigoid, n = 2 2 with linear IgG/IgA deposits on monkey esophagus and salt-split skin; 4 without IR
DH, n = 2 IgA reactivity with endomysium on monkey esophagus and anti-transglutaminase IgA reactivity NS, n = 2 no IR
PNP, n = 2 Both with anti- Dsg3 reactivity and anti envoplakin reactivity PV, n = 2 intercellular IgG/IgA deposits on monkey esophagus and Dsg 3-transfected cells
BP, n = 2 positive BP180NC16A ELISA NS, n = 2 no IR
NS, n = 1 No IR PV, n = 1 intercellular IgG/IgA deposits on monkey esophagus and Dsg 3-transfected cells
  1. In 21 sera (white background), final diagnosis was based on findings by conventional procedures as explained. In 3 sera (grey background), no final diagnosis could be made. In these cases, direct immunofluorescence microscopy would be required.
  2. IR, immunoreactivity; LAD, linear IgA dermatosis; LAD-1, linear IgA dermatosis antigen 1(soluble ectodomain of BP180); p200, p200-pemphigoid; MMP, mucous membrane pemphigoid; DH, dermatitis herpetiformis; BP, bullous pemphigoid; PV, pemphigus vulgaris; NS, normal serum; PNP, paraneoplastic pemphigus; Dsg 3, desmoglein 3.