Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

van Beek, Nina; Rentzsch, Kristin; Probst, Christian; Komorowski, Lars; Kasperkiewicz, Michael; Fechner, Kai; Bloecker, Inga M; Zillikens, Detlef; Stöcker, Winfried; Schmidt, Enno

doi:10.1186/1750-1172-7-49

Orphanet Journal of Rare Diseases

Table 1 Overview of target antigens in immunobullous diseases and diagnostic methods used in this study

From: Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

Disease	Target antigen	Routine multi-step approach¹
Pemphigus vulgaris	Desmoglein 3	Desmoglein 3 ELISA[22]
Pemphigus foliaceus	Desmoglein 1	Desmoglein 1 ELISA[22]
Paraneoplastic pemphigus	Desmoglein 3	Desmoglein 3 ELISA[22]
	Envoplakin	Envoplakin ELISA[21]
	Periplakin/ Desmoplakin I/II	Immunoblot with extract of cultured HaCaT cells[21]
	Periplakin/ Desmoplakin I/II	Indirect IF microscopy on rat and monkey bladder
Bullous pemphigoid	BP180	BP180 NC16A ELISA[20]
	BP230	BP230 ELISA[51]
	Soluble ectodomain of BP180 (LAD-1)	BP180 4575 (c-terminal fragment) Immunoblot[33, 63]
	Soluble ectodomain of BP180 (LAD-1)	LAD-1 Immunoblot[33, 63]
Pemphigoid gestationis	BP180	BP180 NC16A ELISA[20]
Pemphigoid gestationis	BP180	Complement binding test
Linear IgA dermatosis	Soluble ectodomain of BP180 (LAD-1)	Immunoblot with conditioned medium of cultured HaCaT cells (IgA reactivity) )[33, 63]
Linear IgA dermatosis	BP230	BP230 ELISA[51]
Lichen planus pemphigoides	BP180	BP180 NC16A ELISA[51]
Lichen planus pemphigoides	BP230	BP230 ELISA[51]
Mucous membrane pemphigoid	Soluble ectodomain of BP180 (LAD-1)	Immunoblot with conditioned medium of cultured HaCaT cells (IgG and IgA reactivity)[33]
	BP180	BP180 NC16A ELISA[20]
	BP230	BP230 ELISA[51]
	Laminin 332	Immunoblot with extracellular matrix of cultured HaCaT cells[13, 35]
laminin γ1/anti-p200 pemphigoid	p200 protein/ Laminin γ1	Immunoblot with extract of human dermis[34]
laminin γ1/anti-p200 pemphigoid	p200 protein/ Laminin γ1	Immunoblot with recombinant laminin γ1 C-term[37]
Epidermolysis bullosa acquisita	Type VII collagen	Immunoblot with recombinant NC1-domain of type VII collagen[36]
Dermatitis herpetiformis	Epidermal/tissue transglutaminase, gliadin	Transglutaminase ELISA deamidated gliadin-analogous fusion peptide-specific ELISA[32]

¹in addition, all sera were subjected to indirect IF microscopy on human salt-split skin and monkey esophagus. The exact diagnostic algorithm applied in this study is detailed in[38].
IF, immunofluorescence; LAD-1, linear IgA dermatosis antigen 1(soluble ectodomain of BP180); BP180NC16A, targeted extracellular domain of BP180.

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ISSN: 1750-1172

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