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Table 1 Overview of target antigens in immunobullous diseases and diagnostic methods used in this study

From: Serological diagnosis of autoimmune bullous skin diseases: Prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy

Disease Target antigen Routine multi-step approach1
Pemphigus vulgaris Desmoglein 3 Desmoglein 3 ELISA[22]
Pemphigus foliaceus Desmoglein 1 Desmoglein 1 ELISA[22]
Paraneoplastic pemphigus Desmoglein 3 Desmoglein 3 ELISA[22]
Envoplakin Envoplakin ELISA[21]
Periplakin/ Desmoplakin I/II Immunoblot with extract of cultured HaCaT cells[21]
Indirect IF microscopy on rat and monkey bladder
Bullous pemphigoid BP180 BP180 NC16A ELISA[20]
BP230 BP230 ELISA[51]
Soluble ectodomain of BP180 (LAD-1) BP180 4575 (c-terminal fragment) Immunoblot[33, 63]
LAD-1 Immunoblot[33, 63]
Pemphigoid gestationis BP180 BP180 NC16A ELISA[20]
Complement binding test
Linear IgA dermatosis Soluble ectodomain of BP180 (LAD-1) Immunoblot with conditioned medium of cultured HaCaT cells (IgA reactivity) )[33, 63]
BP230 BP230 ELISA[51]
Lichen planus pemphigoides BP180 BP180 NC16A ELISA[51]
BP230 BP230 ELISA[51]
Mucous membrane pemphigoid Soluble ectodomain of BP180 (LAD-1) Immunoblot with conditioned medium of cultured HaCaT cells (IgG and IgA reactivity)[33]
BP180 BP180 NC16A ELISA[20]
BP230 BP230 ELISA[51]
Laminin 332 Immunoblot with extracellular matrix of cultured HaCaT cells[13, 35]
laminin γ1/anti-p200 pemphigoid p200 protein/ Laminin γ1 Immunoblot with extract of human dermis[34]
Immunoblot with recombinant laminin γ1 C-term[37]
Epidermolysis bullosa acquisita Type VII collagen Immunoblot with recombinant NC1-domain of type VII collagen[36]
Dermatitis herpetiformis Epidermal/tissue transglutaminase, gliadin Transglutaminase ELISA deamidated gliadin-analogous fusion peptide-specific ELISA[32]
  1. 1in addition, all sera were subjected to indirect IF microscopy on human salt-split skin and monkey esophagus. The exact diagnostic algorithm applied in this study is detailed in[38].
  2. IF, immunofluorescence; LAD-1, linear IgA dermatosis antigen 1(soluble ectodomain of BP180); BP180NC16A, targeted extracellular domain of BP180.