Subcellular distribution of wild-type and mutant tyrosinase in human melanocytes grown at 31°C. The subcellular localization of GFP-tyrosinase in wild-type and p.Pro21Leu-transfected cells was not significantly different when cells were incubated at 31°C or at 37°C. After transfection with either p.Cys35Arg or p.Tyr411His mutant constructs, the melanocytes grown at 31°C showed an increase in the cytoplasmic vesicular co-localization of the mutant protein with EEA1. For each construct, boxed regions were magnified in the adjacent panels. The scale bar represents 10 um for all panels.