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Table 2 Gene-specific primers for coding regions

From: Novel genetic linkage of rat Sp6 mutation to Amelogenesis imperfecta

Gene Primer sequence
Dlx3 5′-CCAGCATGAGCGGCTCCTTCGATCGCAAGC-3′
  5′-GGTACTCAGTACACAGCCCCAGGGTTAGGC-3′
Dlx4 5′-CCGCAATGACCTCTTTACCCTGTCCCCTTCC-3′
  5′-CAGACTCACATCCTCTGAGGCAGCGCCAGC-3′
Enam 5′-AATAAATGTGTCTTGCTCCTTGGCTCTCTG-3′
  5′- AATACCTAAGCCTGAAGCAGTAAACAGCCG-3′
Fam20a 5′-GGGCCATGCCCGGACTGCGCAGGGACCGCC-3′
  5′-TTGCTGCCGTTAGCTTGTCAGATTAGCCTG-3′
Klk4 5′-CCAATATGATGGTCACTGCACGAACCCCC-3′
  5′-CAGAGCTATCTTGTCTGAATGGTGGTCCAG-3′
Mmp20 5′-AGGAGATGAAGGTGCTACCTGCCTCTGGCC-3′
  5′-ACGATTCAGCAACCAACCCAGGAGCTGG-3′
Ngfr 5′-GTGCAATGAGGAGGGCAGGTGCTGCCTGCAG-3′
  5′-TGAGTTCACACTGGGGATGTGGCAGTGGAC-3′
Sp2 5′-GCAACATGACGCTCACCCTGCCACTCAACAACC-3′
  5′-TGGCCTTACAAGCCCTTCGTGCCTAGGTGGGTC-3′
Sp6 5′-CCGGCAATGCTAACCGCTGTCTGTG-3′
  5′-GGCTCAGTTGGAGGACGCCGAGCTG-3′
a Wdr72(a) 5′-CTAACATGAGGAGTGCTCTGCAGGCTGTGG-3′
  5′-TGCGCATCATCGCCGCAGTT-3′
a Wdr72(b) 5′-GCGTGCCCGGAAGCACCTTT-3′
  5′-TCGGGACTCCATCCATCTAGCAC-3′
  1. aWdr72: We divided the coding region into two fragments (a and b) for cloning.