Figure 5

Effect of α-LA, NAC and α-LA + NAC on chromosome breaks in DEB-induced cultured lymphocytes from FA patients. Lymphocyte cultures were pre-treated with 20 μM α-LA, 500 μM NAC, and both antioxidants simultaneously at the same concentrations, 1.5 h before exposure to DEB 0.05 μg/ml for 48 h. The results are presented as the relative mean of breaks per cell (percentage of control values). Comparison of the relative mean of breaks/cell was made between control group and each antioxidant treatment (*** P < 0.001) and between treatments (P > 0.05). Results are representative of the mean of 14 experiments depicted from Table 4.