Figure 2

Experimental CDH in rats. Administration of 100 mg of the herbicide nitrofen on gestational day 9.5 to pregnant rats induced the malformation in 40 to 80% of the offspring. On the left, neonatal autopsy of an untreated, wild-type rat shows an intact diaphragm (above) that can also be seen in the frontal section of the trunk (below). On the right, similar preparations of a rat with nitrofen-induced CDH show a large postero-lateral diaphragmatic orifice, herniation of the liver into the thorax, displacement of the mediastinum to the contralateral side and lung hypoplasia.