Table 2 Pyrosequencing targets with corresponding Illumina probeset ID and primer used for amplification
Target | Illumina Target ID | Forward primer | Reverse primer | Sequencing primer |
|---|---|---|---|---|
WISP2 | cg03562120 | GTGTGTGTTTGGGAGTGATTT | Bio-CTCATATCCCCTACAAAACCAACTTTAA | GTTTGGGAGTGATTTTATAGTTGT |
HOXA5 | cg02248486 | GGAATTATGATTTTTATAATTATGTAATTGGTAGTT | Bio-AACCACAAATCAAACACACATATCA | AATTATGATTTTTATAATTATGTAATTGGTAG |
HOXA9 | cg27009703 | Bio-GTGGTGATGGTGGTGGTATAT | ACTTCAACCCCTACAACTTCCAATCCA | TCAACCCCTACAACTTCCAATCCAAAA |
WT1 | cg25094569 | Bio-TGGATGTGATTTTGGGATAGGT | CCCATTTTTAAAACCAAACCATTTAACT | ATTTTTAAAAAATAAACAACCTTCTCTATC |
GATA4 | cg17795240 | AAGGATTGGTTTAGGGAGAGTTTGTTTTG | Bio-TAAAATTTCACCATATTAACCAAAAACTCCTAACCTTA | GGTTTAGGGAGAGTTTGTTTTG |