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Figure 2 | Orphanet Journal of Rare Diseases

Figure 2

From: Development of an ELISA for sensitive and specific detection of IgA autoantibodies against BP180 in pemphigoid diseases

Figure 2

Immunoreactivity of IgA autoantibodies with native and recombinant BP180 ectodomain. Precipitated, recombinant (lanes 1-6) and native, keratinocyte-derived (lanes 7-12) BP180 ectodomain immunoblotted with IgA pemphigoid (LAD) patients' sera (lanes 1-3, 7-9) and control sera (NHS) (lanes 4, 5, 10 and 11). As substrate control (lanes 13-18), precipitated culture medium from cells transfected with empty vector was immunoblotted using the same LAD (lanes 13-15) and NHS (lane 16, 17) sera. Presence or absence of the 120 kDa ectodomain was visualized using a specific monoclonal Ab (lanes 6, 12 and 18).

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172