Figure 7
From: Lack of a synergistic effect of a non-viral ALS gene therapy based on BDNF and a TTC fusion molecule
Western blot analysis of proteins involved in the apoptotic and survival signalling pathways. A. Fold-changes in the expression of pro-Casp3 and active Casp3 proteins. B. Bax and Bcl2 proteins and (C) phosphorylated states of Akt and ERK1/2 proteins in spinal cord lysates of control SOD1G93A animals (white) and animals treated with TTC (grey), BDNF-TTC (blue, BTTC) and BDNF (soft blue). Western blot quantities are shown as the ratios to β-tubulin and then related to age-matched wild-type (black) mice data. Significant differences were found in all treated groups compared to control SOD1G93A mice except for pro-Casp3 protein expression in TTC-treated mice. Biochemical behavior of treated transgenic animals resembled that of wild-type age-matched mice (n = 5 mice per group). The normalization of total protein ERK1/2 expression levels as a ratio to β-tubulin yielded a significant increase in the control group (white) with respect to the rest of groups (wild type, TTC-, BDNF- and BDNF-TTC-treated groups). The expression level of total protein of Akt and ERK 1/2 kinases was determined using the housekeeping protein β-tubulin (*P < 0.05 and **P < 0.01 vs. control SOD1G93A mice; *** p < 0.001; error bars indicate SEM).