Figure 2

Effects on extra-cellular concentrations of glutamate. A: comparison of the glutamate concentrations measured before (basal) and after infusion of CSF or purified IgGs of patients (Post-Inf.). Each sample (5 control CSF, 6 patients' CSF with NMDA-R-Ab, one purified IgGs positive for NMDA-R-Ab and one purified IgGs positive for anti-Yo antibodies) was infused in 4 different rats. Means and SDs are represented. Measurements were made at baseline (before infusion), and between 30 and 60 minutes after CSF or purified IgGs infusion. B: point plot (one point corresponds to one rat injected) of the extra-cellular concentrations of glutamate following infusion of CSF (open circles: controls' CSF; filled circles: patients' CSF), and purified IgGs (open circles: control IgGs from a patient with Yo-Ab; filled circles: purified IgGs from a patient positive for NMDA-R-Ab) measured in CA1 zone between 30 and 60 minutes after CSF infusion. C: Top panel: concentrations of extra-cellular glutamate according to the dilution of patients' CSF infused in the CA1 area. Four rats were infused with 3 successive doses from one patient's CSF positive for NMDA-R-Ab (CSF 9049). Linear regression, 95% confidence intervals (dashed lines) and 95% prediction intervals (dotted lines) are shown. Bottom panel: Similar results were obtained with purified IgG of CSF 9052. Four rats were individually infused with 4 successive doses. **: p < 0.01.