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Table 8 Linearity of the reverse transcription and PCR amplification efficiency determinations

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

LCL #

Amplification efficiencies and coefficient of correlation (E/R2)

 

AS1

AS2

HPRT

5

1.882/0.9954

1.988/0.9953

2.059/0.9972

 

2.032/0.9975

2.039/0.9999

2.018/0.9997

6

2.051/0.9965

2.007/0.9998

2.034/0.9994

 

2.010/0.9993

2.014/0.9978

2.019/0.9997

  1. Determinations of the linearity of the reverse transcription reactions are depicted in italics. PCR amplification efficiency determinations are underlined.