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Table 8 Linearity of the reverse transcription and PCR amplification efficiency determinations

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

LCL # Amplification efficiencies and coefficient of correlation (E/R2)
  AS1 AS2 HPRT
5 1.882/0.9954 1.988/0.9953 2.059/0.9972
  2.032/0.9975 2.039/0.9999 2.018/0.9997
6 2.051/0.9965 2.007/0.9998 2.034/0.9994
  2.010/0.9993 2.014/0.9978 2.019/0.9997
  1. Determinations of the linearity of the reverse transcription reactions are depicted in italics. PCR amplification efficiency determinations are underlined.