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Table 5 Intra-assay variability of real-time AS-PCR when screening #1 (n = 3)

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

Experimental run #

AS1

AS2

HPRT

 

Mean Ct

Stdev

CV%

Mean Ct

Stdev

CV%

Mean Ct

Stdev

CV%

1

23.66

0.13

0.54

24.08

0.10

0.40

27.21

0.12

0.42

2

23.65

0.06

0.06

24.13

0.02

0.09

27.16

0.05

0.19

3

23.53

0.27

1.14

24.08

0.11

0.11

27.09

0.12

0.43

  1. Stdev. = Standard deviation, CV%= coefficient of variance.
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Orphanet Journal of Rare Diseases

ISSN: 1750-1172