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Table 5 Intra-assay variability of real-time AS-PCR when screening #1 (n = 3)

From: Allele-specific differences in ryanodine receptor 1 mRNA expression levels may contribute to phenotypic variability in malignant hyperthermia

Experimental run # AS1 AS2 HPRT
  Mean Ct Stdev CV% Mean Ct Stdev CV% Mean Ct Stdev CV%
1 23.66 0.13 0.54 24.08 0.10 0.40 27.21 0.12 0.42
2 23.65 0.06 0.06 24.13 0.02 0.09 27.16 0.05 0.19
3 23.53 0.27 1.14 24.08 0.11 0.11 27.09 0.12 0.43
  1. Stdev. = Standard deviation, CV%= coefficient of variance.