Similar early characteristics but variable neurological outcome of patients with a de novo mutation of KCNQ2

  • Mathieu Milh1, 2Email author,

    Affiliated with

    • Nadia Boutry-Kryza3,

      Affiliated with

      • Julie Sutera-Sardo1, 2,

        Affiliated with

        • Cyril Mignot4, 5, 6,

          Affiliated with

          • Stéphane Auvin7,

            Affiliated with

            • Caroline Lacoste8,

              Affiliated with

              • Nathalie Villeneuve2,

                Affiliated with

                • Agathe Roubertie9, 10,

                  Affiliated with

                  • Bénédicte Heron6,

                    Affiliated with

                    • Maryline Carneiro9,

                      Affiliated with

                      • Anna Kaminska11,

                        Affiliated with

                        • Cécilia Altuzarra12,

                          Affiliated with

                          • Gaëlle Blanchard13,

                            Affiliated with

                            • Dorothée Ville13,

                              Affiliated with

                              • Marie Anne Barthez14,

                                Affiliated with

                                • Delphine Heron4, 5,

                                  Affiliated with

                                  • Domitille Gras7,

                                    Affiliated with

                                    • Alexandra Afenjar5, 6,

                                      Affiliated with

                                      • Nathalie Dorison6,

                                        Affiliated with

                                        • Dianne Doummar6,

                                          Affiliated with

                                          • Thierry Billette de Villemeur5, 6,

                                            Affiliated with

                                            • Isabelle An15,

                                              Affiliated with

                                              • Aurélia Jacquette4,

                                                Affiliated with

                                                • Perrine Charles4,

                                                  Affiliated with

                                                  • Julie Perrier16,

                                                    Affiliated with

                                                    • Bertrand Isidor17,

                                                      Affiliated with

                                                      • Laurent Vercueil18,

                                                        Affiliated with

                                                        • Brigitte Chabrol1, 2,

                                                          Affiliated with

                                                          • Catherine Badens1, 8, 19,

                                                            Affiliated with

                                                            • Gaétan Lesca3 and

                                                              Affiliated with

                                                              • Laurent Villard1, 19

                                                                Affiliated with

                                                                Orphanet Journal of Rare Diseases20138:80

                                                                DOI: 10.1186/1750-1172-8-80

                                                                Received: 8 March 2013

                                                                Accepted: 15 May 2013

                                                                Published: 22 May 2013

                                                                Abstract

                                                                Background

                                                                Early onset epileptic encephalopathies (EOEEs) are dramatic heterogeneous conditions in which aetiology, seizures and/or interictal EEG have a negative impact on neurological development. Several genes have been associated with EOEE and a molecular diagnosis workup is challenging since similar phenotypes are associated with mutations in different genes and since mutations in one given gene can be associated with very different phenotypes. Recently, de novo mutations in KCNQ2, have been found in about 10% of EOEE patients. Our objective was to confirm that KCNQ2 was an important gene to include in the diagnosis workup of EOEEs and to fully describe the clinical and EEG features of mutated patients.

                                                                Methods

                                                                We have screened KCNQ2 in a cohort of 71 patients with an EOEE, without any brain structural abnormality. To be included in the cohort, patient’s epilepsy should begin before three months of age and be associated with abnormal interictal EEG and neurological impairment. Brain MRI should not show any structural abnormality that could account for the epilepsy.

                                                                Results

                                                                Out of those 71 patients, 16 had a de novo mutation in KCNQ2 (23%). Interestingly, in the majority of the cases, the initial epileptic features of these patients were comparable to those previously described in the case of benign familial neonatal epilepsy (BFNE) also caused by KCNQ2 mutations. However, in contrast to BFNE, the interictal background EEG was altered and displayed multifocal spikes or a suppression-burst pattern. The ongoing epilepsy and development were highly variable but overall severe: 15/16 had obvious cognitive impairment, half of the patients became seizure-free, 5/16 could walk before the age of 3 and only 2/16 patient acquired the ability to speak.

                                                                Conclusion

                                                                This study confirms that KCNQ2 is frequently mutated de novo in neonatal onset epileptic encephalopathy. We show here that despite a relatively stereotyped beginning of the condition, the neurological and epileptic evolution is variable.

                                                                Keywords

                                                                Epilepsy Genetics KCNQ2 Encephalopathy

                                                                Background

                                                                KCNQ2 encodes a channel subunit carrying the neuronal Im current whose inherited mutations were first described in autosomal dominant benign familial neonatal epilepsy (BFNE, OMIM#121200) [13]. Patients affected by a BFNE displayed stormy phase of motor seizures during the neonatal period, lasting 2 to 6 weeks in average. Interictal EEG was normal or slightly modified [4]. Subsequently, seizure frequency quickly decreased and the vast majority of patients became seizure free before the age of three months [5]. Motor and cognitive outcome were usually normal. Recently, de novo mutations of KCNQ2 have been described in early onset epileptic encephalopathies (EOEEs; OMIM#613720) [68]. EOEEs are a group of devastating epilepsies beginning before three months of age, with frequent seizures and abnormal interictal EEG leading to a rapid deterioration of motor, cognitive and sensori-neuronal functions. Patients carrying de novo KCNQ2 mutations displayed abnormal interictal EEG that could reveal multifocal spikes or a suppression-burst pattern, and all had poor neurological outcome [7, 8]. This dramatic form of KCNQ2-related epilepsy, with very poor neurological outcome, was unexpected. In order to assess the importance of KCNQ2 screening for the molecular diagnosis of early onset epilepsies, and mostly to describe the outcome of the sporadically mutated patients, we have analyzed a cohort of 71 patients with an early onset, severe epilepsy, without any familial history of epilepsy.

                                                                Methods

                                                                This study was approved by CPP Sud Méditerannée (Comité de protection des personnes). Seventy one patients were included in a cohort of subjects who displayed an early onset epileptic encephalopathy. All the patients or their parents gave their informed consent to join the cohort. Inclusion in the cohort was decided according to the following criteria; (1) epilepsy onset within the first 3 months of age; (2) abnormal interictal EEG (3) brain MRI without obvious cortical malformation or hypoxic lesion; (4) normal metabolic screening (exclusion of nonketotic hyperglycinemia, hyperammonemia, urea cycle defect, organic aciduria, hyperlactacidemia, pyridoxine-dependent and pyridoxal-dependent seizures); (5) No mutation of STXBP1, a major gene involved in early onset epileptic encephalopathy with or without suppression-burst [9]; (6) No mutation of ARX [10] in male patients (n=35); (7) patients must be regularly followed till now. All the girls that displayed early onset epileptic spasms and/or tonic seizures without any suppression-bursts were tested for CDKL5 (n=36). The epilepsy began during the neonatal period for 47/71 patients, the EEG showed a suppression-burst or discontinuous traces in 33 of them (Groupe A), and multifocal spikes in the remaining 14 (Groupe B). Epilepsy began between 1 and 3 months for the 24 patients of groupe C. The 18 coding exons (including alternative exons) of KCNQ2 were sequenced. Primer sequences are available upon request. The identified mutations were numbered according to the KCNQ2 reference sequence NM_172107.2.

                                                                Results and discussion

                                                                We found heterozygous mutations in KCNQ2 in 16/71 patients (Table 1). All of them have occurred de novo. Typically, the first seizure was observed before the 5th day of life (n=14/16), taking the form of clonic and/or tonic seizures resembling those observed in BFNE (12/16). These seizures were very frequent, rapidly leading to obvious neurological impairment before the end of the first week (10/16). Eight patients carrying a KCNQ2 mutation were initially diagnosed with an Ohtahara syndrome, with a typical suppression-burst pattern on the EEG (Table 1, Figure 1). The first EEG did not show any suppression-burst pattern, but discontinuous traces in the remaining patients (Table 1, Figure 1). In three cases, EEGs evolved into a hypsarythmic pattern, but the majority quickly developed into a continuous pattern with multifocal asynchronous spikes and/or slowing of the traces (13/16). The outcome of epilepsy was highly variable: 9/16 patients became seizure free during the follow-up, 6 of them before the end of the first year of life, while 7/16 patients were still epileptic, three of them had only myoclonic jerks, two of them had recurrent generalized tonic clonic seizures and two had focal seizures (Table 1). Fifteen patients had obvious developmental delay: 4/15 could walk but 3/4 had no language and 1/3 had autistic features; 11/15 were profoundly impaired with poor or absent head control and eye contact (8/15) or global/axial hypotonia with poor or absent hand use (3/15). One patient had a good evolution with normal neurological evaluation at age 6. The initial brain MRI was normal or showed very slight and transitory brain signal abnormalities in 12/16 patients, while in 3 patients, abnormal signal intensity were found, as previously described [7] (Table 2). Only one patient had extra-neurological features: congenital left hip luxation and cleft palate (patient 2). Fifteen patients had a mutated KCNQ2 in the group A (45%, n=33), one patient had a mutation of KCNQ2 in the group B (7%, n=14) and none of the patient had a KCNQ2 mutation in the group C. Thus, KCNQ2 was mutated in half of patients with a neonatal onset epileptic encephalopathy and an EEG showing either discontinuous or suppression-burst pattern. Here, we confirm that, besides well described entity BFNE, KCNQ2 mutations can also be associated with severe epileptic and cognitive phenotypes defining early onset epileptic encephalopathies [7]. Hence, it should be considered in the diagnosis workup of neonatal onset epilepsies especially those beginning during the first week of life with stormy clonic and/or tonic seizures, whatever the presence or absence of a familial history. If cognitive outcome is relatively reliable and good in familial cases of BFNE [5], it is not the case in sporadic ones, where neurological outcomes range from dramatic to normal. We did not find any relationship between the initial history of the epilepsy and the severity of outcome. For example, patients 1 and 12 had relatively similar features at the beginning and displayed very different outcomes (Tables 1, 2 and Figure 1). Since KCNQ2 is now implicated in various forms of epilepsies, from the most benign to the most dramatic, additionnal data on phenotype/genotype correlations would be particularly relevant. Interestingly, none of the mutation reported in neonatal epileptic encephalopathies had previously been reported in BFNE, and the severe mutations that have been found in several patients (p.G290A [7], p.T274M and p.A294V(present study)) lead to relatively similar features in terms of initial EEG and development, however different in terms of evolution of the epilepsy. The different epileptic features in patients carrying the same mutation of KCNQ2 may be due to genetic modifiers or non genetic factors. Overall, this cohort of patients highlights the heterogeneous evolution of the neurological phenotypes associated with de novo heterozygous mutations in KCNQ2. This heterogeneity could be at least partially related to the impact of the mutations on the Im current. Analysis of the functional consequences of “benign” versus “severe” mutations in KCNQ2 should be of paramount importance to better understand the molecular and cellular mechanisms involved in the emergence of an epileptic encephalopathy. This has recently been tested with two mutations of KCNQ2 affecting the same residue in the S4 domain of the protein KV7-2 but associated with either a benign phenotype, or with a neonatal epileptic encephalopathy with severe drug-resistant seizures and neurocognitive delay, suppression-burst pattern at EEG, and distinct neuroradiological features [11]. The authors showed that, while both mutations destabilized the open state of the channel causing a reduction of the voltage sensitivity, the functional changes were more pronounced in the “severe” mutation than in the benign one. In both cases, the functional impairment could be fully restored by the neuronal Kv7 activator retigabine. This study suggested that the clinical disease severity may be related to the extent of the mutation-induced functional K+ channel impairment and set the preclinical basis for the potential use of Kv7 openers as a targeted anticonvulsant therapy to improve developmental outcome in neonates. However, since two patients carrying the same KCNQ2 mutation do not have the same epileptic outcome, correlations between Im impairment and the severity of the encephalopathy should be made with caution. Other unknown factors may be involved in the occurrence of the epileptic encephalopathy. Moreover, the vast majority of the mutations we described here were localised on segment S6 and should have different consequences on Im current that those which have been previously described, affecting segment S4 [11]. These consequences still have to be studied. Overall, it is not known whether ongoing brain dysfunction that is observed in several patients is due to the Kv7-2 channelopathy or if it is a sequel of neonatal epilepsy. This question would be of paramount interest.
                                                                Table 1

                                                                KCNQ2 mutations and main features of the patients

                                                                 

                                                                Mutation

                                                                Seizure onset (days)

                                                                Initial seizure type

                                                                Seizure evolution

                                                                First EEG

                                                                Development (age at evaluation)

                                                                Patient 1

                                                                c.C860A p.T287N

                                                                1

                                                                Clonic and tonic. Multiple seizures daily.

                                                                2 weeks: seizure offset.

                                                                Suppression-burst.

                                                                Poor eye contact, poor head control (9 months). Normal HC.

                                                                Patient 2

                                                                c.G523T p.V175L

                                                                15

                                                                Myoclonic jerks. No erratic myoclonus.

                                                                0-3 months: myoclonic jerks.

                                                                Discontinuous.

                                                                Deceased at 17 months.

                                                                Bursts of polyspikes generalized or in the in central regions.

                                                                No eye contact, no head control. HC : 41cm.

                                                                3–6 months: reflex audiogenic seizures.

                                                                6–12 months: epileptic spasms.

                                                                >12 months: myoclonic jerks.

                                                                Patient 3

                                                                c.C926T p.A309V

                                                                3

                                                                Tonic, pallor, Multiple seizures daily.

                                                                0-24 months: multiple daily focal seizures. 2–5 years: 1 seizure/week. 7 years: epilepsy offset.

                                                                Suppression-burst.

                                                                Poor eye contact. Global hypotonia, unable to sit (2 years)

                                                                Patient 4

                                                                c.C821T p.T274M

                                                                2

                                                                Tonic and hypotonic. Epileptic spasms.

                                                                2 months: seizure free. Erratic intermittent myoclonus.

                                                                Suppression-burst.

                                                                Poor eye contact, no head control, global hypotonia (14 years). Normal HC.

                                                                Right temporal, asymptomatic seizures.

                                                                Patient 5

                                                                c.G715C p.G239R

                                                                2

                                                                Tonic and tonic-clonic, cyanosis.

                                                                2-6 weeks: Tonic and tonic-clonic seizures in clusters. 2 m: seizure stop

                                                                Poor activity. Prolonged periods of flatness of the traces. Generalized spikes predominating on the left hemisphere. Then suppression-burst.

                                                                Good eye contact. Sitting, hand use (10 months). Walking (22 months).

                                                                No speech (4 y) Normal HC

                                                                Patient 6

                                                                c.C881T p.A294V

                                                                2

                                                                Left and right clonic jerks, facial cyanosis.

                                                                3 months: Seizure offset.

                                                                Suppression-burst.

                                                                Poor head control, unable to sit, no voluntary movement, no language (2 years).

                                                                Patient 7

                                                                c.C881T p.A294V

                                                                1

                                                                Isolated access of cyanosis. Then recurrent hypertonic posture.

                                                                7 months: epileptic spasms.

                                                                Suppression-burst.

                                                                Eye contact. Strabismus.

                                                                No sit, no speech (11 y).

                                                                Multiple focal seizures: tonic contractions of one or several limbs, cyanosis.

                                                                2–9 years: seizure-free.

                                                                > 9 years: monthly GTC seizures.

                                                                Patient 8

                                                                c.T911C p.F304S

                                                                1

                                                                Tonic asymmetric.

                                                                2 months: multifocal seizures. 4 months: rhythmic jerks. 3 years: tonic seizures, cyanosis. 3-11y: Persistence of tonic seizures, cyanosis.

                                                                Bursts of multifocal spikes and periods of poorness of the activity.

                                                                Unable to sit, poor use of hands. No language. Feeding difficulties (gastrostomy, 11 years)

                                                                Patient 9

                                                                c.G566T p.G189V

                                                                3

                                                                Tonic.

                                                                0-6 months: multiple focal seizures. 6–24 months: epileptic spasms. Seizure free since then.

                                                                Suppression-burst.

                                                                Poor eye contact. Global hypotonia, unable to sit (10 years)

                                                                Patient 10

                                                                c.G793A p.A265T

                                                                1

                                                                Tonic and/or clonic, Multiple seizures daily.

                                                                >4 months: myoclonic jerks

                                                                Burst of asynchronous spikes and sharp waves. Periods of discontinuity with flatness of the traces without classical suppression burst.

                                                                Poor eye contact. Global hypotonia, poor head control, pyramidal signs (6 months)

                                                                Patient 11

                                                                c.A886C p.T296P

                                                                1

                                                                Tonic, cyanosis, Multiple seizures daily.

                                                                0-19 days: multiple seizures. 6-18 m: no seizure. 18 m- 11y: several episodes of CTGC or PS with secondary generalization.

                                                                Left or right spikes on a moderately abnormal background.

                                                                Walking (18 months). Poor language, autistic features (11 years)

                                                                Patient 12

                                                                c.2318dupG p.C774Lfs*91

                                                                4

                                                                Partial motor seizure with asymmetric tonic extension of one limb. Bilateral clonic seizures. Apnea.

                                                                1 month: seizure free.

                                                                Suppression-burst.

                                                                Slight peripheral hypertonia (3 months). Good outcome, walking (18 months), normal language (5 years)

                                                                Patient 13

                                                                c.G471A p.W157X

                                                                4

                                                                Hemi corporeal, left or right.

                                                                0-11 months: partial clonic seizures. Then seizure offset.

                                                                Poor, discontinuous.

                                                                Independent walking (4 y). No language (6.5 y). Normal HC 52.5 cm

                                                                Patient 14

                                                                c.G868A p.G290S

                                                                1

                                                                Tonic.

                                                                Many motor seizures during the neonatal period. 2 m: Seizure stop. AED withdrawn at 4 years.

                                                                Asymmetrical suppression-burst with multifocal slow waves, left frontal and right occipital spikes. Periods of generalized flattening.

                                                                Sitting (3 y) hand stereotypies. Unable to walk/stand, stereotypies, pyramidal signs. Poor language. Normal HC (16 y).

                                                                Patient 15

                                                                c.C881T p.A294V

                                                                8

                                                                Myoclonic jerks, Multiple seizures daily.

                                                                0-3 months: myoclonic jerks. 3 months: seizure offset. Therapy stopped at 6 months.

                                                                Suppression burst.

                                                                Sit (2 y). No walking, 2–3 words. Understands simple orders. Strabismus, nystagmus (3 y)

                                                                Patient 16

                                                                c.997C>T. p.R333W

                                                                2

                                                                Bilateral tonic clonic And right clonic

                                                                0-3 y: active epilepsy, motor seizures 3-10 y: seizure free 10-20 y: monthly focal seizures

                                                                Slow waves with asynchronous bilateral spikes and intermittent flattening

                                                                First steps (18 m). Few words (3 y) Able to read but cannot write, limited communication skills, marked bradypsychia, hand stereotypies (26 y)

                                                                HC head circumference, m months, y year.

                                                                http://static-content.springer.com/image/art%3A10.1186%2F1750-1172-8-80/MediaObjects/13023_2013_579_Fig1_HTML.jpg
                                                                Figure 1

                                                                Representative early EEGs of patients carrying a de novo KCNQ2 mutation. A. Interictal EEG (Day 3, patient 1), showing a typical suppression-burst pattern, with burst of spikes and slow waves alternating with periods of electric silence (left panel). Sometimes, the burst should be much longer than the periods of suppression, leading to a discontinuous pattern (Right panel). B. EEG displaying the same features (Patient 12, suppression-burst in left panel, discontinuous pattern in right one), with a very different outcome (normal development at 5 years old, see Table 1).

                                                                Table 2

                                                                Data on initial evaluation and treatment, EEG evolution and brain MRI

                                                                 

                                                                Term. Clinical examination at birth

                                                                Treatment during the first month

                                                                EEG evolution (age)

                                                                Brain MRI (age)

                                                                Patient 1

                                                                Full term. Hypotonia. No eye contact. BW: 3000 g HC: 35 cm

                                                                PHB VGB

                                                                1 m: Continuous with rare posterior spikes and fast rhythms

                                                                Day 7: Normal, absence of any signal abnormality. 2 y: Discrete global brain atrophy, thin corpus callosum

                                                                6 m: slow background, rare focal spikes.

                                                                Patient 2

                                                                34 GW Fetal distress, apnea, movements disorder BW: 2,040 g HC: 30 cm

                                                                MDZ, PHB, B6, TPM, VGB

                                                                0-7 m: Suppression-burst >7 m: Hypsarythmia

                                                                D13: absence of any signal abnormality. 3 m: Absence of signal abnormality

                                                                Patient 3

                                                                Full term Failure. to thrive. Feeding difficulties BW: 3,770 g HC: 37.5 cm

                                                                PHB, B6, PHT, VGB, TPM, CLB.

                                                                1-6 w: Asynchronous SB. 2-8 m: bilateral Bursts of central spikes 1-6 y: Bursts of rhythmic generalized spikes at 3 Hz

                                                                Day 3: T1 bilateral hypersignal of pallida, tegmentum, locus niger, hippocampi. Abnormal ADC in these regions. 2 y: T1 hypersignal of the same structures and diffuse T1 hypersignal of the white matter. Brain atrophy

                                                                Patient 4

                                                                Full term. Normal BW: 3,580 g HC: 37 cm

                                                                PHB, PHT, VPA

                                                                0-2 m: Suppression-burst. 2–12 m: hypsarythmia. >12 m: Frequent multifocal spikes

                                                                Day 7: Normal CT scan 2 y: Thin Corpus Callosum, absence of signal abnormality

                                                                Patient 5

                                                                Full term. Normal BW: 3,240 g HC: 34 cm

                                                                PHB, PHT, B6, VGB, VPA

                                                                0-2 m: Suppression-burst. 2–6 m: continuous, slow background, multifocal spikes. >6 m: Rare spikes in temporal and occipital lobes

                                                                Day 10: no signal abnormality.

                                                                Patient 6

                                                                Full term. Normal BW: 2,790 g HC: 34,5 cm

                                                                ND

                                                                0-1 m: Suppression-burst. <1 m: Multifocal spikes, slow background

                                                                1 m: Normal

                                                                Patient 7

                                                                Full term. Normal BW: 3,180 g, HC: 36 cm

                                                                PHB, B6, PHT

                                                                0-1 m: Suppression-burst. 1–7 m: Continuous EEG, multifocal spikes 7–24 m: Hypsarythmic pattern. >24 m: Frequent spikes and spike wave in frontal regions

                                                                Day 4: Normal, absence of any signal abnormality

                                                                Patient 8

                                                                ND

                                                                PHB, PHT

                                                                0-2 m: Bursts of multifocal spikes, periods of flatness. >2 m: Multifocal spikes, poor organization

                                                                1 y: No structural or signal abnormality.

                                                                Patient 9

                                                                At term. Hypotonia. No eye contact. BW: 3,450 g HC: 35 cm

                                                                PB, CZP, VGB

                                                                0-2 m: Suppression-burst 2–6 m: Slow background, rare generalized spike waves. 6–12 m: Hypsarythmic pattern. >12 m: Rare asynchronous frontal and temporal spikes

                                                                Day 10: Normal, absence of any signal abnormality

                                                                Patient 10

                                                                Full term. No eye contact BW: 3,120 g HC: 33 cm

                                                                PB, PHT, TPM, VGB, B6,

                                                                0-2 m: discontinuous EEG. >2 m: continuous, slow EEG with rare generalized spikes

                                                                Day 5: T1: symmetrical hypersignal of the pallida, caudate nuclei and hippocampi T2: bilateral hypersignal of the parietal occipital white matter

                                                                Patient 11

                                                                Full term. Hypotonia, hyporeactivity, failure to feed

                                                                PHB, PHT, VPA.

                                                                0-1 m: Left or right spikes on a moderately abnormal background. >1 m: Occipital or temporal spikes with left prominence with progressive migration on the central temporal region

                                                                1 m: normal

                                                                Patient 12

                                                                Full term. Normal BW and HC

                                                                VGB, CBZ

                                                                0-2 m: Suppression-burst. 2–6 m: General slowing of the traces, no spike. 6 m-2 y: Rare spikes in the right central region, Normal background. >2 y: normal traces.

                                                                Day 7: T2 hyperintensity of the basal ganglia 2 y: Normal 3y: Normal

                                                                Patient 13

                                                                Full term. Fetal distress. BW, HC: ND

                                                                ND

                                                                ND

                                                                1 m: No structural abormality, no signal change

                                                                Patient 14

                                                                Full term. BW 3,750 g. Poor eye contact, trunk hypotonia with bouts of hypertonia

                                                                PHB, VGB, CBZ;

                                                                0-4 m: Asymmetrical suppression-burst 4-10 m: Left occipital spikes and slow waves 10 m-3 y: Normal background activity + posterior theta waves, No spike 3 y: Intermittent slow background, no spike >8 y: Normal

                                                                3 m: Normal

                                                                Patient 15

                                                                Oligoamnios Born at 30 Weeks (GA) BW: 1580 g HC: 29 cm

                                                                PHB, VPA

                                                                0-1 m: Suppression-burst >1 m: continuous traces (normal)

                                                                2 y: normal

                                                                Patient 16

                                                                Full term Global hypotonia, weak cry

                                                                CLN, PHT

                                                                0-1 m: absence of physiologic features, slow waves, spikes, brief flattening. 1–6 m: improvement of background activity, some generalized flattening episodes, left occipital slow waves. 6 m – 6 y: slow. background activity, rare spikes. 27 y: bilateral temporal slow waves. 30 y: normal background activity, bilateral fronto-temporal bursts of slow waves, photic stimulation-evoked slow spikes

                                                                17 y: slight T2 and FLAIR hyperintensity of thalami.

                                                                GW gestational week, HC Head circumference, m month, Y year, PHB Phenobarbital, PHT phenytoine, VGB vigabatrin, TPM topiramate, CLN clonazepam, VPA Sodium Valproate, CBZ carbamazepine, CLB clobazam.

                                                                Conclusions

                                                                KCNQ2 is frequently found mutated de novo in early onset epileptic encephalopathies, especially if the epilepsy begins within the first week of life. Despite relatively stereotyped initial phenotype, the neurological and epileptic outcomes were highly variable, overall severe.

                                                                Declarations

                                                                Acknowledgements

                                                                This work was supported by INSERM (Contrat d'Interface pour Hospitalier to MM), Programme Hospitalier de recherche Clinique, Aix Marseille Université and Assistance Publique Hôpitaux de Marseille (Contrat Hospitalier de Recherche Translationnelle to LV). We thank the Centre de Ressources Biologiques of La Timone Children’s Hospital for access to the biological samples used in this study.

                                                                Authors’ Affiliations

                                                                (1)
                                                                INSERM, UMR_S 910 Faculté de médecine, Boulevard jean MOULIN F13005
                                                                (2)
                                                                APHM, Service de neurologie pédiatrique, CHU Timone
                                                                (3)
                                                                Hospices civils de Lyon. Laboratoire de génétique, Hôpital Edouard Herriot. Bron
                                                                (4)
                                                                APHP, Unité Fonctionnelle de Génétique Médicale, Département de Génétique, Groupe Hospitalier Pitié-Salpêtrière
                                                                (5)
                                                                Centre de Référence des Déficiences Intellectuelles de Causes Rares
                                                                (6)
                                                                APHP. Service de Neuropédiatrie, Hôpital Armand Trousseau
                                                                (7)
                                                                APHP. Service de neuropédiatrie, Hopital Robert Debré
                                                                (8)
                                                                APHM. Département de Génétique Médicale et Biologie Cellulaire CHU Timone
                                                                (9)
                                                                CHU Montpellier. Service de neuropédiatrie
                                                                (10)
                                                                INSERM U1051, INM Montpellier
                                                                (11)
                                                                APHP. Service de neurophysiologie clinique Hôpital Necker
                                                                (12)
                                                                CHU Besancon. Service de neuropédiatrie
                                                                (13)
                                                                Hospices civils de Lyon, Service de neuropédiatrie. HFME. Bron
                                                                (14)
                                                                CHU de Tours. Service de neuropédiatrie
                                                                (15)
                                                                APHP. Groupe hospitalier Pitié Salpétrière. Service de neurologie
                                                                (16)
                                                                CHU de Nantes. Service de pédiatrie
                                                                (17)
                                                                CHU de Nantes. Service de génétique médicale
                                                                (18)
                                                                CHU de Grenoble. Service d’électrophysiologie clinique
                                                                (19)
                                                                Faculté de Médecine, Aix Marseille Université

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                                                                © Milh et al.; licensee BioMed Central Ltd. 2013

                                                                This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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