Copper and bezafibrate cooperate to rescue cytochrome c oxidase deficiency in cells of patients with sco2 mutations
- Alberto Casarin1,
- Gianpietro Giorgi1,
- Vanessa Pertegato1,
- Roberta Siviero2,
- Cristina Cerqua1,
- Mara Doimo1,
- Giuseppe Basso3,
- Sabrina Sacconi4,
- Matteo Cassina1,
- Rosario Rizzuto2,
- Sonja Brosel5,
- Mercy M Davidson5,
- Salvatore DiMauro5,
- Eric A Schon5, 6,
- Maurizio Clementi1,
- Eva Trevisson†1 and
- Leonardo Salviati†1Email author
© Casarin et al.; licensee BioMed Central Ltd. 2012
Received: 17 November 2011
Accepted: 15 March 2012
Published: 19 April 2012
Mutations in SCO2 cause cytochrome c oxidase deficiency (COX) and a fatal infantile cardioencephalomyopathy. SCO2 encodes a protein involved in COX copper metabolism; supplementation with copper salts rescues the defect in patients’ cells. Bezafibrate (BZF), an approved hypolipidemic agent, ameliorates the COX deficiency in mice with mutations in COX10, another COX-assembly gene.
We have investigated the effect of BZF and copper in cells with SCO2 mutations using spectrophotometric methods to analyse respiratory chain activities and a luciferase assay to measure ATP production..
Individual mitochondrial enzymes displayed different responses to BZF. COX activity increased by about 40% above basal levels (both in controls and patients), with SCO2 cells reaching 75-80% COX activity compared to untreated controls. The increase in COX was paralleled by an increase in ATP production. The effect was dose-dependent: it was negligible with 100 μM BZF, and peaked at 400 μM BZF. Higher BZF concentrations were associated with a relative decline of COX activity, indicating that the therapeutic range of this drug is very narrow. Combined treatment with 100 μM CuCl2 and 200 μM BZF (which are only marginally effective when administered individually) achieved complete rescue of COX activity in SCO2 cells.
These data are crucial to design therapeutic trials for this otherwise fatal disorder. The additive effect of copper and BZF will allow to employ lower doses of each drug and to reduce their potential toxic effects. The exact mechanism of action of BZF remains to be determined.
KeywordsCOX deficiency Bezafibrate SCO2 Copper chaperones Copper supplementation
Cytochrome c oxidase (COX), complex IV of the mitochondrial respiratory chain (RC), is comprised of 13 structural subunits and a number of prosthetic groups and metal cofactors . For its biogenesis, COX requires several ancillary proteins encoded by COX assembly genes, which are needed for the synthesis of the prosthetic groups, for the delivery of the metal cofactors, and for the stabilization of the nascent polypeptides . Mutations in COX assembly genes (mainly SURF1, SCO2, and COX10) are the most common cause of isolated COX deficiency . These disorders are characterized by severe encephalomyopathy, and involvement of other tissues (hypertrophic cardiomyopathy in SCO2 patients , tubulopathy and/or cardiomyopathy in COX10 patients ), and usually leads to death in infancy or early childhood. There is no established therapy for these conditions.
COX10 is required for heme biosynthesis , while the precise function of SCO2 and SURF1 is still debated. Homologues of SURF1 in bacteria appear to function as heme-binding proteins , while SCO2 is implicated in copper metabolism although its function is not completely clear [4, 8]. SCO2 itself is a copper-binding protein, and it has been shown that copper supplementation can rescue COX deficiency in cells harboring mutations in this gene [9, 10]. Although a preliminary trial in patients yielded promising results , and supplementation with copper salts is currently employed in patients with Menkes disease , the potential toxicity of these compounds is a major hindrance to their therapeutic use.
A novel approach for the treatment of RC disorders is based on induction of mitochondrial biogenesis. This was achieved in experimental models by overexpression of PPARgamma -coactivator alpha (PGC-1alpha), or by pharmacological treatment. Bezafibrate (BZF), an approved hypolipidemic agent, was originally employed in patients with disorders of mitochondrial fatty acid metabolism with promising results . It was later shown to be effective also in RC disorders. Mice with mitochondrial myopathy due to muscle-specific knockout of the COX10 gene displayed increased muscle COX activity and ATP levels, delayed onset of myopathy, and markedly prolonged life span . BZF proved effective also in cells of patients with COX deficiency . Another approach is based on 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR). This compound acts by activating AMP-activated protein kinase , which in turn stimulates PGC-1alpha expression. AICAR was found to be effective in mice with mutations in SURF1 SCO2, and COX15 , whereas BZF was not effective in mice and was associated with significant toxicity in both control and COX-deficient animals. However, the response to BZF is a species specific phenomenon, and hepatomegaly after BZF treatment is typical of rodents but is not usually seen in other mammalian species, including humans [18, 19]. Moreover, AICAR is still not approved for human use, while BZF is routinely employed in clinical practice. We therefore examined the effect of BZF on a cellular model of SCO2 deficiency to see if this drug could have a role for the treatment of patients with SCO2 mutations.
Materials and methods
Reagents and cell lines
Cell culture reagents were purchased from Invitrogen. All other chemicals were purchased from Sigma.
Cell lines used in this work have been described previously . Briefly, Pt 1 and Pt 2 were compound heterozygotes for the common SCO2 mutation E140K and for a truncating mutation. Pt 3 was a compound heterozygote for two truncating SURF1 mutations. Patients with coenzyme Q10 deficiency have been described previously .
Cell lines were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen) supplemented either with 20% fetal bovine serum (FBS) (primary skin fibroblasts) or with 10% FBS (all other cell lines), 100 mg/mL streptomycin, and 100 units/mL penicillin.
Copper and BZF supplementation
BZF was solubilised in DMSO to obtain a 100 mM stock solution. This stock solution was further diluted with DMSO to the desired concentration. The total dose of DMSO solvent in the culture medium was 0.5% for all samples (i.e. 60 μL in 12 mL of medium). CuCl2 was mixed in the medium before the addition of serum in order to avoid precipitation. Cells were treated with BZF alone for five days, whereas BZF + CuCl2 supplementation experiments were carried out for 10 days .
RC complexes were analyzed using standard spectrophotometric assays as described [21, 22]. Results were normalized to total protein, and to citrate synthase (CS). However, in copper supplementation experiments, activities were normalized only to protein, because CS activity is inhibited by high copper concentrations . Ornithine aminotransferase (OAT) activity was measured as described . Statistical significance was calculated as previously reported .
Equal amounts of total cell proteins were separated by PAGE using 4-12% gradient gels and tris-glycine buffer (Invitrogen). After blotting, membranes were probed either with an antibody against COXII (Molecular Probes) or with an antibody against SCO2 (Santa Cruz), and with an anti actin antibody (Sigma) according to the manufacturer’s protocol. Detection was performed using the ECL advanced kit (GE biosciences). Densitometry was performed using the ImageJ software.
Luciferase assay was carried out, as previously described . In brief, wild type or SCO2 mutant fibroblasts (200,000-300,000 per coverslip) were grown in the presence or absence of 400 μM BZF for four days. They were then transfected with mitochondrial targeted luciferase (mtLUC) using the Amaxa Nucleofector apparatus (Lonza) and a standard electroporation procedure. The cells were then treated for additional 24 h. The coverslips were transferred to the 37°C thermostated chamber of a luminometer and perfused with a Krebs Ringer Buffer containing: 125 mM NaCl, 5 mM KCl, 1 mM Na3PO4, 1 mM MgSO4, 20 μM Luciferin, 20 mM Hepes, 5,5 mM Glucose (pH7,4). Luminescence is entirely dependent on the continuously provided luciferin and proportional to ATP concentration (between 20 and 200 μM). After 60 s equilibration in the new medium, during which the light emission of mitochondrial luciferase-transfected cells was in the range of 500–5000 cps versus a background lower than 10 cps, cellular response was evoked by adding the agonist histamine (100 μM) to the perfusion medium.
Cell death and ROS measurements
4.5x104 cells grown in 12-well plates were treated with BZF at the indicated concentration and after 4 days treated with 1 mM H2O2 for 2 h . Cells were stained with propidium iodide (PI) and annexin-V-FITC (Prodotti Gianni) and cell death was measured by flow cytometry as the percentage annexin-V, PI-positive cells . ROS production was assayed using dichlorodihydrofluorescein diacetate and flow cytometry as described .
BZF stimulates the activity of individual RC complexes
BZF induces comparable increase of COX activity in different cell lines
Next, we focused on the effect on COX in different cell lines: HEK293 cells, primary skin fibroblasts from a healthy individual, and bone marrow-derived SEM cells. We detected similar increases of COX activity (+37%, +38%, and +43%) in the three cell types compared to untreated samples (Figure 1B). In all cases, COX activity was virtually 100% KCN-sensitive. Again we did not detect significant variations of CS activity.
BZF stimulates COX activity in SCO2 cells in a dose-dependent but peaks at 400 μM
We then analysed the effect of BZF in normal and COX-deficient fibroblasts. Although this cell model is not ideal because fibroblasts display only partial COX deficiency (COX activity around 50% of controls), MyoD-transformed fibroblasts, myoblasts or myotubes obtained from these patients also displayed only partial COX deficiency  and we detected the full COX-deficient phenotype only in fully differentiated muscle. A similar phenomenon was noted with other assembly factors such as FOXRED1 . Therefore these cell types do not provide significant advantages compared to skin fibroblasts for our studies.
We confirmed the lack of stimulation of complex II in cultured skin fibroblasts and we did not detect any effect on CoQ10 content in CoQ10 deficient fibroblasts (not shown).
BZF increases ATP production in fibroblasts with SCO2 mutations
A mitochondrially targeted chimera of ATP-sensitive photoprotein Luciferase (mtLUC) was exploited to dynamically monitor ATP synthesis within the mitochondrial subcellular compartment. The method is based on the reaction of luciferase with the substrate luciferin in presence of oxygen, as the resulting light emission is a linear function of ATP concentration in a range between 10-3 and 10-2 M. SCO2 fibroblasts and normal controls were grown for 5 days in the presence of 400 μM BZF (see methods). ATP production was triggered by addition of 100 μM histamine to the medium.
We then evaluated the effect of BZF treatment. In control cells, there was a reduction of the ATP increase after stimulation with histamine (+10.5± 2% of the basal levels) compared to untreated cells, (Figure 6A, Tr2), similar to what has been observed in cells overexpressing PGC- 1alpha, which display a reduction of Ca2+ transients in the mitochondrial matrix  with reduced stimulation of matrix dehydrogenases and a less evident rise in ATP levels upon stimulation. In SCO2 mutant cells, we observed the opposite effect, as BZF treatment caused a significant increase in ATP levels (+10.4 ± 2% of basal levels) after stimulation with histamine (Figure 6B, Tr3). These data suggest that the biochemical defect was significantly restored, with a detectable effect on the functional output of mitochondria.
BZF and CuCl2 display an additive effect in rescuing COX deficiency in SCO2 cells
Effect of CuCl2 or BZF treatment on SCO2 protein levels
With the exception of coenzyme Q10 deficiency , there is currently no established treatment for patients with RC defects. Pharmacological stimulation of the biogenesis of the RC is a promising new approach for the treatment of these disorders. Two compounds have been shown to be effective to this purpose. BZF, which has been successfully employed in patients with defects of mitochondrial beta-oxidation, was shown to be effective also in a mouse model of COX deficiency due to COX10 mutations , and in cells of patients with COX deficiency due to COX10 mutation and with unknown defects. However a recent study could not confirm the efficacy of BZF in mice with defects in SCO2 COX15, or SURF1, and it was found to cause severe hepatomegaly in both control and COX-deficient animals, whereas AICAR, another activator of the PPARgamma/PGC-1alpha pathway, could rescue the phenotype in these animals .
Yet, these negative results concerning the efficacy of BZF should be taken with caution for two reasons. Mice (and rodents in general) are not a good model to study BZF. In fact it has been shown that there are marked species-specific differences in the effects of BZF, which is known to cause hepatomegaly in mice and rats, but not in other mammals such as dogs and monkeys  (or humans). The biological bases of this phenomenon are not clear. In fact, BZF is commonly used in patients and elevation of liver enzymes is an infrequent side effect in clinical use . Moreover, our results show that the therapeutic window for BZF is very narrow (see below), therefore dosage is a critical issue when administering BZF to animals (or patients), and excessive doses may result in lack of response. A possible explanation for the observed lack of effectiveness of BZF in the COX deficient mice models, could be that the plasma levels of the drug fell outside the narrow therapeutic range (animals were administered BZF in drinking water and serum levels were not monitored). As pointed out by Djouadi and Bastin mice received a dose of BZF 100-fold the therapeutic dose used in patients .
Although the effects of AICAR in COX deficient mice were striking, this compound is still not approved for medical use, while BZF and copper are routinely used in patients. We have therefore examined the effectiveness of BZF (with or without copper) in human cells with SCO2 deficiency, to assess the potential use of these drugs in patients with SCO2 mutations. Our data support the efficacy of the combined treatment with these two compounds. Although in principle we cannot rule out the hypothesis that the effect we observed occurs only in cultured cells, and not in whole tissues, nevertheless patients with beta oxidation defects receiving BZF therapy displayed an increase in COX activity in their muscles . Our results highlight some important points that should be taken into account when designing experiments in non rodent animal models, or therapeutic trials in patients.
First, the response of COX activity to BZF treatment was similar (an increase of about 40% compared to baseline levels) in all cell lines we studied (primary fibroblasts, HeLa, HEK293, and SEM cells), and the same relative increase was also detected in COX-deficient cells (both in SCO2 and SURF1 mutants), indicating that BZF does not increase efficiency of the assembly process, but simply stimulates COX biogenesis as a whole. The increase of COX activity after BZF treatment was accompanied by increase of cellular ATP production in SCO2 cells.
However, the precise mechanism of action of BZF is unclear . In fact we detected an increase in enzymatic activities of complex I, III, and IV, whereas other mitochondrial proteins, both matrix enzymes such as CS and OAT, or localized to the mitochondrial inner membrane, such as complex II and SCO2 protein itself, were unaffected by BZF treatment, ruling out an effect of BZF on mitochondrial biogenesis as a whole. Coenzyme Q10 levels in deficient cells were similarly unaffected. BZF appears to be stimulating only RC complexes which contain mitochondrial DNA encoded subunits, and not mitochondrial biogenesis as a whole. These findings argue against an involvement of the PGC1alpha pathway, but the exact mechanism of action remains to be elucidated. The apparently different mechanism of action of AICAR and BZF suggest that these two compound could also be used in synergy. Future work will be aimed to test this hypothesis.
Second, we noted that the effect of BZF on COX activity peaks at a BZF concentration of 400 μM in the culture medium. However, higher BZF concentrations (600 μM) do not result in a plateau of the effect, but we observed instead a relative reduction of COX activity. A slight decrease of COX activity with 500 μM BZF was in fact noted also by Bastin and coworkers  and they did not test higher BZF doses. These results are critical for therapeutic trials, because they imply that plasma BZF levels must be closely monitored in patients, since the therapeutic window for this compound appears to be very narrow, and doses too high or too low will be ineffective. The reason for the observed decrease in efficacy at higher BZF concentrations is not clear. We observed a minor increase in susceptibility to apoptosis in cells treated with 600 μM BZF, but only after incubation with H2O2. Some sort of negative feedback mechanism could be acting, but further work is needed to address this issue.
Third, and most important, we demonstrated a synergistic effect between copper and BZF. The effect of BZF alone is relatively modest, but we had shown previously that CuCl2 can rescue COX activity in SCO2 mutant cells in a dose-dependent manner with complete recovery of COX activity at 200 μM CuCl2 . Preliminary trials in patients have also yielded promising results, although one major drawback is the toxicity of copper . Our present data demonstrate that CuCl2 and BZF have an additive effect, which permits to employ lower doses of each compound and still achieve complete normalization of COX activity in patient’s cells. It will be possible to avoid at least part of the toxicity related to high serum copper levels, while employing BZF at doses lower than the peak effective levels of 400 μM.
Taken together, our data support the use of BZF in combination with copper in patients with SCO2 mutations. We underscore the fact that both compounds are routinely used in patients with other diseases therefore their utilization for SCO2 patients would simply represent an off-label use. We also stress the importance for clinicians, in particular pediatric neurologists and cardiologists, to consider the possibility of SCO2 mutations in newborns and infants presenting with hypertrophic cardiomyopathy of unexplained origin. Early diagnosis is essential for the ultimate outcome of patients because, as we learned from children with CoQ10 deficiency , effective treatments may stop the progression of the neurological disease but will not affect the already established cerebral lesions.
cytochrome c oxidase
PPARgamma -coactivator alpha
peroxisome proliferator-activated receptor
This work was supported by Telethon Grants GGP06256 and GGP09207 (to L.S.), and GPP1005 (to R.R.), and by grants from Fondazione CARIPARO- Padova (to L.S. and R.R), the National Institute of Health (HD 32062) and the Marriott Mitochondrial Disorder Clinical Research Fund (to S.DM), and from NICHD (P01 HD 32062) and the Muscular Dystrophy Association (To E.A.S.).
- Kadenbach B, Jarausch J, Hartmann R, Merle P: Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure. Anal Biochem. 1983, 129 (2): 517-521.View ArticlePubMedGoogle Scholar
- Barrientos A, Gouget K, Horn D, Soto IC, Fontanesi F: Suppression mechanisms of COX assembly defects in yeast and human: Insights into the COX assembly process. Biochim Biophys Acta. 2009, 1793 (1): 97-107.PubMed CentralView ArticlePubMedGoogle Scholar
- Sacconi S, Salviati L, Sue CM, Shanske S, Davidson MM, Bonilla E, Naini AB, De Vivo DC, DiMauro S: Mutation screening in patients with isolated cytochrome c oxidase deficiency. Pediatr Res. 2003, 53 (2): 224-230.View ArticlePubMedGoogle Scholar
- Papadopoulou LC, Sue CM, Davidson MM, Tanji K, Nishino I, Sadlock JE, Krishna S, Walker W, Selby J, Glerum DM, et al: Fatal infantile cardioencephalomyopathy with COX deficiency and mutations in SCO2, a COX assembly gene. Nat Genet. 1999, 23 (3): 333-337.View ArticlePubMedGoogle Scholar
- Antonicka H, Leary SC, Guercin GH, Agar JN, Horvath R, Kennaway NG, Harding CO, Jaksch M, Shoubridge EA: Mutations in COX10 result in a defect in mitochondrial heme A biosynthesis and account for multiple, early-onset clinical phenotypes associated with isolated COX deficiency. Hum Mol Genet. 2003, 12 (20): 2693-2702.View ArticlePubMedGoogle Scholar
- Glerum DM, Tzagoloff A: Isolation of a human cDNA for heme A:farnesyltransferase by functional complementation of a yeast cox10 mutant. Proc Natl Acad Sci U S A. 1994, 91 (18): 8452-8456.PubMed CentralView ArticlePubMedGoogle Scholar
- Bundschuh FA, Hannappel A, Anderka O, Ludwig B: Surf1, associated with Leigh syndrome in humans, is a heme-binding protein in bacterial oxidase biogenesis. J Biol Chem. 2009, 284 (38): 25735-25741.PubMed CentralView ArticlePubMedGoogle Scholar
- Leary SC, Sasarman F, Nishimura T, Shoubridge EA: Human SCO2 is required for the synthesis of CO II and as a thiol-disulphide oxidoreductase for SCO1. Hum Mol Genet. 2009, 18 (12): 2230-2240.View ArticlePubMedGoogle Scholar
- Jaksch M, Paret C, Stucka R, Horn N, Muller-Hocker J, Horvath R, Trepesch N, Stecker G, Freisinger P, Thirion C, et al: Cytochrome c oxidase deficiency due to mutations in SCO2, encoding a mitochondrial copper-binding protein, is rescued by copper in human myoblasts. Hum Mol Genet. 2001, 10 (26): 3025-3035.View ArticlePubMedGoogle Scholar
- Salviati L, Hernandez-Rosa E, Walker WF, Sacconi S, DiMauro S, Schon EA, Davidson MM: Copper supplementation restores cytochrome c oxidase activity in cultured cells from patients with SCO2 mutations. Biochem J. 2002, 363 (Pt 2): 321-327.PubMed CentralView ArticlePubMedGoogle Scholar
- Freisinger P, Horvath R, Macmillan C, Peters J, Jaksch M: Reversion of hypertrophic cardiomyopathy in a patient with deficiency of the mitochondrial copper binding protein Sco2: is there a potential effect of copper?. J Inherit Metab Dis. 2004, 27 (1): 67-79.View ArticlePubMedGoogle Scholar
- Sherwood G, Sarkar B, Kortsak AS: Copper histidinate therapy in Menkes' disease: prevention of progressive neurodegeneration. J Inherit Metab Dis. 1989, 12 (Suppl 2): 393-396.PubMedGoogle Scholar
- Bonnefont JP, Bastin J, Behin A, Djouadi F: Bezafibrate for an inborn mitochondrial beta-oxidation defect. N Engl J Med. 2009, 360 (8): 838-840.View ArticlePubMedGoogle Scholar
- Wenz T, Diaz F, Spiegelman BM, Moraes CT: Activation of the PPAR/PGC-1alpha pathway prevents a bioenergetic deficit and effectively improves a mitochondrial myopathy phenotype. Cell Metab. 2008, 8 (3): 249-256.PubMed CentralView ArticlePubMedGoogle Scholar
- Bastin J, Aubey F, Rotig A, Munnich A, Djouadi F: Activation of peroxisome proliferator-activated receptor pathway stimulates the mitochondrial respiratory chain and can correct deficiencies in patients' cells lacking its components. J Clin Endocrinol Metab. 2008, 93 (4): 1433-1441.View ArticlePubMedGoogle Scholar
- Corton JM, Gillespie JG, Hawley SA: 5-aminoimidazole-4-carboxamide ribonucleoside. A specific method for activating AMP-activated protein kinase in intact cells?. Eur J Biochem. 1995, 229 (2): 558-565.View ArticlePubMedGoogle Scholar
- Viscomi C, Bottani E, Civiletto G, Cerutti R, Moggio M, Fagiolari G, Schon EA, Lamperti C, Zeviani M: In vivo correction of COX deficiency by activation of the AMPK/PGC-1alpha axis. Cell Metab. 2011, 14 (1): 80-90.PubMed CentralView ArticlePubMedGoogle Scholar
- Watanabe T, Horie S, Yamada J, Isaji M, Nishigaki T, Naito J, Suga T: Species differences in the effects of bezafibrate, a hypolipidemic agent, on hepatic peroxisome-associated enzymes. Biochem Pharmacol. 1989, 38 (2): 367-371.View ArticlePubMedGoogle Scholar
- Goldenberg I, Benderly M, Goldbourt U: Update on the use of fibrates: focus on bezafibrate. Vasc Health Risk Manag. 2008, 4 (1): 131-141.PubMed CentralView ArticlePubMedGoogle Scholar
- Montero R, Sanchez-Alcazar JA, Briones P, Hernandez AR, Cordero MD, Trevisson E, Salviati L, Pineda M, Garcia-Cazorla A, Navas P, et al: Analysis of Coenzyme Q10 in muscle and fibroblasts for the diagnosis of CoQ10 deficiency syndromes. Clin Biochem. 2008, 41 (9): 697-700.View ArticlePubMedGoogle Scholar
- Sacconi S, Salviati L, Nishigaki Y, Walker WF, Hernandez-Rosa E, Trevisson E, Delplace S, Desnuelle C, Shanske S, Hirano M, et al: A functionally dominant mitochondrial DNA mutation. Hum Mol Genet. 2008, 17 (12): 1814-1820.PubMed CentralView ArticlePubMedGoogle Scholar
- Spinazzi M, Casarin A, Pertegato V, Ermani M, Salviati L, Angelini C: Optimization of respiratory chain enzymatic assays in muscle for the diagnosis of mitochondrial disorders. Mitochondrion. 2011, 11 (6): 893-904.View ArticlePubMedGoogle Scholar
- Dekaney CM, Wu G, Jaeger LA: Ornithine aminotransferase messenger RNA expression and enzymatic activity in fetal porcine intestine. Pediatr Res. 2001, 50 (1): 104-109.View ArticlePubMedGoogle Scholar
- Jouaville LS, Pinton P, Bastianutto C, Rutter GA, Rizzuto R: Regulation of mitochondrial ATP synthesis by calcium: evidence for a long-term metabolic priming. Proc Natl Acad Sci U S A. 1999, 96 (24): 13807-13812.PubMed CentralView ArticlePubMedGoogle Scholar
- Viola G, Fortunato E, Cecconet L, Disaro S, Basso G: Induction of apoptosis in Jurkat cells by photoexcited psoralen derivatives: Implication of mitochondrial dysfunctions and caspases activation. Toxicol In Vitro. 2007, 21 (2): 211-216.View ArticlePubMedGoogle Scholar
- Eruslanov E, Kusmartsev S: Identification of ROS using oxidized DCFDA and flow-cytometry. Methods Mol Biol. 2010, 594: 57-72.View ArticlePubMedGoogle Scholar
- Salviati L, Sacconi S, Mancuso M, Otaegui D, Camano P, Marina A, Rabinowitz S, Shiffman R, Thompson K, Wilson CM, et al: Mitochondrial DNA depletion and dGK gene mutations. Ann Neurol. 2002, 52 (3): 311-317.View ArticlePubMedGoogle Scholar
- Fassone E, Duncan AJ, Taanman JW, Pagnamenta AT, Sadowski MI, Holand T, Qasim W, Rutland P, Calvo SE, Mootha VK, et al: FOXRED1, encoding an FAD-dependent oxidoreductase complex-I-specific molecular chaperone, is mutated in infantile-onset mitochondrial encephalopathy. Hum Mol Genet. 2010, 19 (24): 4837-4847.PubMed CentralView ArticlePubMedGoogle Scholar
- Brini M, Pinton P, King MP, Davidson M, Schon EA, Rizzuto R: A calcium signaling defect in the pathogenesis of a mitochondrial DNA inherited oxidative phosphorylation deficiency. Nat Med. 1999, 5 (8): 951-954.View ArticlePubMedGoogle Scholar
- Bianchi K, Vandecasteele G, Carli C, Romagnoli A, Szabadkai G, Rizzuto R: Regulation of Ca2+ signalling and Ca2+−mediated cell death by the transcriptional coactivator PGC-1alpha. Cell Death Differ. 2006, 13 (4): 586-596.View ArticlePubMedGoogle Scholar
- Foltopoulou PF, Zachariadis GA, Politou AS, Tsiftsoglou AS, Papadopoulou LC: Human recombinant mutated forms of the mitochondrial COX assembly Sco2 protein differ from wild-type in physical state and copper binding capacity. Mol Genet Metab. 2004, 81 (3): 225-236.View ArticlePubMedGoogle Scholar
- Trevisson E, DiMauro S, Navas P, Salviati L: Coenzyme Q deficiency in muscle. Curr Opin Neurol. 2011, 24 (5): 449-456.View ArticlePubMedGoogle Scholar
- Djouadi F, Bastin J: Species differences in the effects of bezafibrate as a potential treatment of mitochondrial disorders. Cell Metab. 2011, 14 (6): 715-716. author reply 717View ArticlePubMedGoogle Scholar
- Viscomi C, Zeviani M: Response to Drs. Djouadi and Bastin. Cell Metab. 2011, 14 (6): 717.View ArticleGoogle Scholar
- Montini G, Malaventura C, Salviati L: Early coenzyme Q10 supplementation in primary coenzyme Q10 deficiency. N Engl J Med. 2008, 358 (26): 2849-2850.View ArticlePubMedGoogle Scholar
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