SP-D counteracts GM-CSF-mediated increase of granuloma formation by alveolar macrophages in lysinuric protein intolerance
© Douda et al; licensee BioMed Central Ltd. 2009
Received: 7 July 2009
Accepted: 23 December 2009
Published: 23 December 2009
Pulmonary alveolar proteinosis (PAP) is a syndrome with multiple etiologies and is often deadly in lysinuric protein intolerance (LPI). At present, PAP is treated by whole lung lavage or with granulocyte/monocyte colony stimulating factor (GM-CSF); however, the effectiveness of GM-CSF in treating LPI associated PAP is uncertain. We hypothesized that GM-CSF and surfactant protein D (SP-D) would enhance the clearance of proteins and dying cells that are typically present in the airways of PAP lungs.
Cells and cell-free supernatant of therapeutic bronchoalveolar lavage fluid (BALF) of a two-year-old patient with LPI were isolated on multiple occasions. Diagnostic BALF samples from an age-matched patient with bronchitis or adult PAP patients were used as controls. SP-D and total protein content of the supernatants were determined by BCA assays and Western blots, respectively. Cholesterol content was determined by a calorimetic assay or Oil Red O staining of cytospin preparations. The cells and surfactant lipids were also analyzed by transmission electron microscopy. Uptake of Alexa-647 conjugated BSA and DiI-labelled apoptotic Jurkat T-cells by BAL cells were studied separately in the presence or absence of SP-D (1 μg/ml) and/or GM-CSF (10 ng/ml), ex vivo. Specimens were analyzed by light and fluorescence microscopy.
Here we show that large amounts of cholesterol, and large numbers of cholesterol crystals, dying cells, and lipid-laden foamy alveolar macrophages were present in the airways of the LPI patient. Although SP-D is present, its bioavailability is low in the airways. SP-D was partially degraded and entrapped in the unusual surfactant lipid tubules with circular lattice, in vivo. We also show that supplementing SP-D and GM-CSF increases the uptake of protein and dying cells by healthy LPI alveolar macrophages, ex vivo. Serendipitously, we found that these cells spontaneously generated granulomas, ex vivo, and GM-CSF treatment drastically increased the number of granulomas whereas SP-D treatment counteracted the adverse effect of GM-CSF.
We propose that increased GM-CSF and decreased bioavailability of SP-D may promote granuloma formation in LPI, and GM-CSF may not be suitable for treating PAP in LPI. To improve the lung condition of LPI patients with PAP, it would be useful to explore alternative therapies for increasing dead cell clearance while decreasing cholesterol content in the airways.
Pulmonary alveolar proteinosis (PAP) syndrome is thought to occur due to multiple causes including genetic defects [1–3], immune deficiencies , malignancies [5, 6] and infection . Patients with PAP have milky alveolar infiltrates that often contain excess surfactant material and large numbers of white blood cells. PAP is usually treated by whole lung lavage (WLL) [8–12] or with GM-CSF administration [13–16]. GM-CSF is a hematopoietic growth factor known to stimulate stem cells to proliferate into granulocytes or monocytes , promote differentiation of monocytes into alveolar macrophages [18–20], and increase the catabolism within alveolar macrophages [21, 22], and increase the innate immune potential of neutrophils . Therefore, GM-CSF is an important cytokine that could regulate PAP at multiple levels.
PAP-like condition is also reported in surfactant protein D (SP-D) deficient mice [24–26]. SP-D is an innate immune collectin that is present in lungs and other mucosal surfaces . It opsonizes pathogens and dying cells, and enhances their uptake by the alveolar macrophages [28, 29]. SP-D deficient mice also accumulate lipid-laden foamy macrophages [25, 26] and apoptotic cells in their lungs . Moreover, transgenic over-expression of SP-D  or administration of recombinant fragments of SP-D  has been shown to enhance immune function in the lungs in mice. Therefore, SP-D and GM-CSF could significantly enhance the innate immune functions of alveolar macrophages.
PAP is one of the deadliest phenotypes seen in Lysinuric protein intolerance (LPI) [32–34]. LPI is an autosomal recessive disorder characterized by mutations in the SLC7A7 (solute carrier family 7, member 7) gene, which encodes a dibasic cationic amino acid transporter, y+LAT1 [35, 36]. Mutant SLC7A7 proteins cause defective transport of the cationic amino acids arginine, lysine, and ornithine [35–38]. PAP develops in some LPI patients and appears to be different from idiopathic PAP . PAP in LPI presents with large numbers of cholesterol crystals and granulomas [32, 33, 39]. The precise cause or appropriate treatment options for the clinical presentation of PAP in LPI is currently unknown. Since LPI is not a well-characterized disease, and has multiple symptoms , it may have also been misdiagnosed as other conditions, and hence, the prevalence of this disease may be higher than the reported numbers . Therefore, it is important to study the disease phenotypes and treatment options. We hypothesized that defective clearance of materials present in the airways contributes to the presentation of PAP in LPI patients, and treating the LPI cells with SP-D and GM-CSF could enhance the innate immune potential of these cells.
Consistent with our hypothesis, we found that SP-D and GM-CSF increase the uptake of proteins and dying cells by LPI AMs. Unexpectedly, we found that the LPI AMs spontaneously formed granulomas, ex vivo. Moreover, while control cells remained unchanged, the addition of GM-CSF to the LPI AM cultures resulted in a marked increase in the number of granulomatous structures. Notably, SP-D counteracted the negative effect of GM-CSF. In conclusion, although GM-CSF may have therapeutic advantage in certain types of PAPs, it may not be suitable for treating PAP of the LPI patients.
Materials and methods
SLC7A7 gene mutation analysis was performed by Dr. Ginafranco Sebastio in Naples, Italy. ABCA3, SP-B and SP-C genes were sequenced and analyzed by Ambrey Genetics.
All buffer salts and reagents were obtained from Sigma unless otherwise stated. SP-D was isolated from BALF of an adult PAP patient as described previously [40, 41]. Recombinant GM-CSF expressed in yeast (Leukine) was produced by Berlex (Seattle, WA). All cell culture media were obtained from Invitrogen (Carlsbad, CA).
Collection of WLL and BALF
Therapeutic WLL was performed in the LPI patient or idiopathic PAP patient, and the BALF samples were colleted in 100 ml vials. Diagnostic BALF sample was also obtained from another airway disease patient. BALF was filtered with nylon mesh and centrifuged at 200 × g for 10 min at 4°C. The pellets containing the cells were then washed twice with Hank's Buffered Salt Solution (HBSS). The supernatant was centrifuged again at 10,000 × g for 40 min at 4°C to collect surfactant lipid pellets, which were stored at -80°C until further analyses.
Analysis of the BALF
BCA® Protein Assay kit from Pierce (Rockford, IL) was used for measuring the protein concentrations in the pre-filtered BALF from each collection vial according to the manufacturer's instructions. SP-D concentration levels and degradation were assessed in the BALF from the LPI patient by Western blotting using anti-human SP-D rabbit sera as described previously . An aliquot of the BALF from each collectin tube was immediately mixed with 0.2% (w/v) Trypan Blue solution and counted using a Hemocytometer at 20× or 40× normal magnification. The amount of cholesterol present in the BALF samples were assessed using the Cholesterol Assay Kit (Cayman Chemical Co., Ann Arbor, MI).
Cytospin preparations were made using 50 and 100 μl BALF, cells were air dried and subjected to Hemacolor® staining (EMD Chemicals, Inc, Gibbstown, NJ) for histological analysis. Other cytospin preparations were fixed with 2% (v/v) paraformaldehyde (PFA) in PBS for 1 h at 4°C, and were subjected to staining with Oil Red O (Polysciences, Inc, Washington, PA) for lipid analysis of foamy macrophage cells. Oil Red O stock solution was prepared by dissolving 0.5% (w/v) Oil Red O powder in isopropanol at 37°C. This stock solution was then diluted to 60% (v/v) with dH2O to make the working solution. Cytospin preparation of cells were rinsed with 60% (v/v) isopropanol, and stained with freshly prepared Oil Red O working solution for 15 min and counterstained with Hemacolor Solution 3 for nuclei. Cells were imaged using Leitz Laborlux D microscope with Leica IM 50 image manager software.
Cell pellets and surfactant lipid pellets obtained from 200 × g and 10,000 × g centrifugation of BALF, respectively, were fixed for at least 18 h at 4°C with 2% (v/v) paraformaldehyde (PFA) and 4% (v/v) glutaraldehyde (Electron Microscopy Sciences, Fort Washington, PA) in a buffer containing 50 mM HEPES (pH 7.4), 150 mM NaCl, and 1 mM CaCl2. Samples were then processed, stained with 2% (w/v) uranyl acetate and analyzed by transmission electron microscopy (JEOL TEM 1011) with AMT Image Capture Engine software at 2,000-40,000 times normal magnification.
Cell pellets obtained from 200 × g centrifugation of the BALF were immediately re-suspended in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% (v/v) heat-inactivated Fetal Bovine Serum (FBS) with 100 U/ml penicillin, 100 μg/ml streptomycin, and 2.5 μg/ml Fungezone. Cells were then allowed to adhere to chamber slides or 24- or 48-well culture plates for 1 h, and non-adherent cells were washed. Cells were cultured in the same media, and subsequently, the media was changed to Macrophage Serum-Free Media (MSFM), a specific media available for culturing macrophage cells (Invitrogen), and does not require supplementation with serum, prior to the experiments. The Jurkat T cell line was maintained in RPMI containing 10% (v/v) FBS.
Induction of apoptosis
Jurkat T cells were first labelled with 2 μl/ml DiI membrane dye (Invitrogen) in MSFM for 4 min at 37°C, followed by three washes with HBSS. Apoptosis was induced by UVC irradiation for 30 s using UV Stratalinker 2400 (120 J/cm2). Cells were then placed in the humidified incubator at 37°C for 2 h in the absence or presence of SP-D (5 μg/ml).
BSA/Apopotic cell uptake assay
Cells were incubated first in MSFM overnight prior to the experiment. Cells were then treated with SP-D (1 μg/ml) for 4 h, and GM-CSF (10 ng/ml) for 30 min prior to the experiment. Alexa-647 conjugated BSA was added to the culture for 30 min, at which point the cells were fixed with 2% (v/v) PFA for 10 min at room temperature, followed by fresh 2% (v/v) PFA for 1 h at 4°C. DiI-labelled apoptotic Jurkat T cell uptake assay was performed in the same manner as BSA using the Jurkat cells 2 h after the induction of apoptosis (1:5 of apoptotic cells:macrophages).
Fluorescence microscopy and image analysis
Images for the uptake assays, and granuloma assay were captured using Zeiss Axiovert 200 M fluorescence microscope with Volocity software. Measurement of cell area, color (label-specific gray levels) and size was performed using Volocity software.
Granuloma formation assay
Cells were incubated in the presence or absence of SP-D (1 μg/ml) and/or GM-CSF (10 ng/ml) for 29 days ex vivo. Culture media was replaced with a fresh media with the addition of appropriate reagents (SP-D and/or GM-CSF) every 3-5 days. After 28 days, the granulomas started to dislodge from the substratum.
A two-tailed student's t-test was performed using Microsoft Excel on Oil Red O positive cell counting and apoptotic Jurkat cell uptake analysis. Protein uptake assay, and cell shape and size were analyzed using Dunnett's multiple mean comparison tests using JMP statistical analysis software (Version 5.0; SAS Institute Inc.). A p-value was set at 0.05 for statistical significance. Non-linear regression analysis and comparisons of regression lines by ANOVA and F-test was performed using GraphPad PRISM statistical analysis software (Version 4.0; GraphPad Software).
LPI and control patients
A Canadian boy with Dutch ancestry was diagnosed with LPI at 9 months of age. His symptoms included feeding difficulty, vomiting, hepatomegaly and failure to thrive. Symptoms improved after initiating dietary therapy with protein restriction and citrulline supplementation. However, a progressive productive cough developed by age 18 months. Chest X-rays showed an interstitial pneumonitis pattern and bronchiolar lavage fluid confirmed the presence of Periodic acid-Schiff (PAS) stain positive staining alveolar macrophages consistent with the diagnosis of PAP. The patient, however, did not have mutations in ABCA3, SP-B, or SP-C. Despite appropriate dietary therapy and treatment of gastro-esophageal reflux, the boy's respiratory symptoms progressed and he developed a persistent oxygen need. A trial of systemic steroid therapy (2 mg/kg body weight) was ineffective. At 2 1/2 years of age referral was made to our pediatric respiratory medicine service. Since WLL is associated with significant morbidity and technically difficult in small children, a three-month trial of inhaled GM-CSF (250 μg once daily delivered through a pari-star nebulizer with a mouthpiece) was instituted. The boy's respiratory deterioration continued. Therefore, a WLL procedure was performed after the discontinuation of GM-CSF treatment. After the procedure, there seemed to be some initial clinical improvement, but the respiratory symptoms relapsed within 3 to 5 days. His disease progressed to the point of respiratory failure requiring intubation and mechanical ventilation despite two further lung lavages. A lung biopsy only confirmed the diagnosis of PAP and interstitial pneumonitis secondary to LPI. The presence of cholesterol granulomas in the airways was also confirmed by the biopsy. The boy was unresponsive to further courses of high dose pulse intravenous corticosteroid therapy and eventually died.
Diagnostic BALF samples from a patient who had bronchitis of unknown etiology and no specific airways disease were used as a control. In some experiments, we also used BALF samples obtained from adult idiopathic PAP patient.
BALF contains high levels of protein and dying cells
Reduced bioavailability of SP-D in LPI PAP surfactant
The role of SP-D in human PAP is not well understood. Using multiple LPI PAP lavage samples from the patient, we first determined whether there were any alterations in SP-D. Western blots detected SP-D that was either modified or tightly associated with other components present in the airways (upward smear) as well degraded SP-D (low molecular weight bands) (Figure 1D). This suggested that the bioavailability of functional SP-D would be low in these airways. To further assess this point, we analyzed the surfactant lipids isolated from the supernatant obtained after removing the cell pellet. The surfactant lipid isolation was performed by a subsequent centrifugation of this supernatant at high-speed (10,000 × g). We first examined the surfactant lipid structure in detail. Lattice-like tubular myelin structure was detected in the surfactant lipid (Figure 1E, arrowhead). There were also many amorphous electron-dense structures present (Figure 1E, arrow). Notably, the PAP surfactant also contained unusual forms of tubular myelin with circular lattices (Figure 1F). This circular tubular myelin had the cross-sectional diameter of approximately 90 nm, with electron dense "spot" in its centre, a structure similar to that of SP-D mediated tubular myelin . These unusual structures indicated that SP-D is likely trapped inside these tubular structures. The presence of atypical circular tubular myelin and the cleaved/modified collectin suggested that the bioavailability of SP-D would be low in these airways.
LPI PAP BALF contains abundant cholesterol and foamy cells
To characterize the different types of immune cells present in the airways, cytospin preparations were made from BALF, fixed and stained with Oil Red O, a dye that primarily stains neutral lipids, triglycerides and lipoproteins (Figure 2C). While few cells from bronchitis control BALF were Oil Red O positive, a significantly higher percentage of cells stained positive in the LPI BALF indicating the presence of lipid-laden foamy macrophage cells (> 20-fold; p < 0.05; Figure 2C). To further confirm the nature of these cells, cells were isolated from BALF samples and examined using electron microscopy. Large numbers of vacuolated foamy macrophages, other immune cells, small-sized apoptotic cells with condensed nuclei and cellular debris were readily detected (Figure 2D). Furthermore, foamy cells that contain cholesterol crystals within their cytoplasm were often observed (Figure 2E). Large cholesterol needles were also present outside the cells in these preparations. Collectively, these results demonstrated that the LPI patient had PAP with multiple alterations potentially impairing lung function.
LPI alveolar macrophage cells respond to SP-D and GM-CSF during uptake of protein and apoptotic cells
SP-D deficiency results in the accumulation of apoptotic cells, in vivo , and PAP airways contain increased number of dead cells (Figures. 1 and 2). Thus, we next asked whether SP-D and/or GM-CSF could enhance the clearance of apoptotic cells by the cells isolated from the LPI BALF (Figure 3C and 3E). These cells were incubated with apoptotic Jurkat T cells for 4 h in the presence/absence of SP-D and/or GM-CSF. SP-D was pre-incubated with apoptotic cells after the induction of apoptosis with UV irradiation. We manually quantified the images of cells as either "Uptake" where the cells have taken up apoptotic cell material, and "No Uptake". Manual counting of randomly selected images (double blinded) revealed that, like BSA uptake, the uptake of apoptotic cells/bodies was significantly increased when macrophages were incubated with SP-D coated apoptotic cells in the presence of GM-CSF (p < 0.05; Figure 3C).
SP-D and GM-CSF affect the cell size and shape
During the course of our experiments, we serendipitously discovered that the BAL cells began to progressively extend long processes, especially those cultured ex vivo for longer than 7 days. In agreement with this phenomenon, the cell size increased with increase in number of days (Figure 4F). Because of the change in cell shape and size, we asked whether there was an effect on the ability of these cells to internalize BSA. Quantifying BSA uptake revealed that the proportion of cells participating in the uptake of BSA decreased over time, and was inversely related to cell size (Figure 4F).
GM-CSF increases granulomatous structure formation
PAP in LPI is often untreatable and results in death. In this study, we carefully characterized BALF and tissue samples obtained from an LPI patient. The data revealed elevated levels of protein and dying cells in the airways. In addition, large cholesterol crystals and abnormal tubular myelin structures were found. Our primary cell culture assays using Alexa-647 conjugated BSA and apoptotic Jurkat T cells showed that pre-incubation of the LPI BAL cells with SP-D and GM-CSF increased their innate immune functions. Surprisingly, many of these LPI cells, but not the control cells, became elongated and stopped internalizing foreign material; eventually they formed granulomas ex vivo. Notably, treating these cells with GM-CSF ex vivo dramatically increased granuloma formation. However, treating the cells with SP-D reduced GM-CSF-mediated granuloma formation. Therefore, these findings may provide important clues for devising better treatment of PAP in LPI patients.
Although various SLC7A7 mutations can cause LPI, the importance of different mutations in the disease phenotype is not clearly established . LPI is relatively frequent in Finland and Italy [46, 47], and in Japan, 1:119 individuals are heterozygous carriers for this LPI gene mutations with estimated frequency of 1:50000 individuals living with LPI . LPI has also been reported in other countries where variable penetrance of specific founder mutations was shown to be responsible for the disease [37, 49]; hence, many LPI individuals with SLC7A7 mutations may be misdiagnosed.
This is the first report that shows LPI in a patient with Dutch ancestry. Consistent with the definition of PAP [1, 12, 27], this patient's BALF had PAS positive material (data not shown) and >100-fold protein levels compared to control, as well as increased numbers of dead cells (Figure 1). High amount of cholesterol and several cholesterol crystals were also seen (Figure. 2). These pathological indices are consistent with reduced lung function seen in these patients.
Although Western blot analyses showed that SP-D was present in the BALF, some SP-D was modified or tightly bound to other components. Furthermore, a considerable amount of SP-D was partially cleaved (Figure 1D). These conditions would render SP-D less functional [50, 51]. Furthermore, electron microscopy analysis of the surfactant lipid pellets showed alterations in tubular myelin structures; notably, the tubular myelin had circular lattice structures, as opposed to the SP-A-mediated square lattice-like structures that are typically seen in healthy humans (Figure 1E and 1F) [43, 52]. Tubular myelin with circular lattice structures is not reported in normal human lungs, but could be generated with SP-D and phosphatidylinositol, in vitro . Since these lipid structures could trap SP-D, together with the fact that there was degraded SP-D, we suggest that the bioavailability of free SP-D is limited in the airways of PAP lungs.
Electron microscopy analysis further revealed the presence of large numbers of cholesterol crystals in the surfactant consistent with previous reports on LPI with PAP [32, 33, 39]. Since the cholesterol level is elevated in airways and serum of idiopathic PAP patients , cholesterol may be one of the important contributors of the pathology in all types of PAP. High cholesterol levels in the lung surfactant increases surface tension [54, 55], and thus may reduce overall lung function. Interestingly, lovastatin, a cholesterol-reducing drug, has been shown to increase apoptotic cell clearance . Therefore, we suggest that it would be worthwhile to explore the possibility of lowering cholesterol in the lungs while increasing dead cell clearance. This may be an alternative treatment option for reducing pulmonary complications in LPI patients.
Since GM-CSF [2, 3, 22, 23, 57–62] and SP-D deficiency [24–26, 42] cause a PAP-like phenotype, we examined their potential therapeutic role ex vivo. Although both GM-CSF and SP-D are usually present in the lungs, it is important to determine whether the BAL cells isolated from the patient respond to these proteins. We were able to show a positive effect of GM-CSF and SP-D in the uptake of protein and apoptotic cells (Figure 3). These results are consistent with the roles of GM-CSF and SP-D suggested by in vivo mouse experiments [30, 63, 64]. Therefore, our results show that, similar to wild type cells, "healthy" LPI cells can respond to SP-D and GM-CSF effectively.
These healthy viable LPI cells however also formed granulomatous structure ex vivo (Figure. 5). Within about one week, many of these cells become elongated and large. They stopped internalizing foreign materials regardless of the presence or absence of SP-D or GM-CSF (Figure 5). Some of the cells formed elongated fibers and connected with each other whereas others migrated to a common location and formed granulomatous structures. It is logical to consider that defective surfactant homeostasis [1–7, 22–26, 42, 57–62, 65–68], accumulation of dead/dying cells  and foamy macrophages [21, 25, 26, 69], and increased granulomas  would reduce lung function in the patients with PAP and LPI. Therefore, alternative strategies are necessary to treat PAP in these patients.
Aerosol [70, 71] and subcutaneous [14, 72] GM-CSF therapy have been successful in treating some patients with PAP [14, 70–72]. Overexpression of GM-CSF in mouse [20, 73] and rat [74, 75] lungs has been shown to increase the recruitment of monocytes [20, 75] and proliferation  and differentiation of alveolar macrophages . Under certain conditions, GM-CSF also increases granuloma formation  and fibrosis  in the lung. Subcutaneous GM-CSF therapy (6 μg/kg per day) has been tried for treating PAP in another child with LPI, but was unsuccessful because the administration of GM-CSF had side effects and lead to excessive leukocytosis . A recent report described a successful therapeutic WLL on a 10-year-old Italian boy with LPI who presented with PAP . The pulmonary condition in the patient described in our report, however, did not improve after WLL, and the patient eventually died of pulmonary insufficiency.
Our experiments using the primary cells isolated from the LPI patient did not confirm the effectiveness of GM-CSF alone in protein or apoptotic cell uptake assays; however, when GM-CSF was added together with SP-D, the innate immune potential of these LPI cells were enhanced. GM-CSF also showed a remarkable increase in granulomatous structure formation, but SP-D was able to reduce the negative effect of this cytokine. Taken together, our results suggest that although GM-CSF is a possible therapeutic molecule for treating patients with autoimmune idiopathic PAP, it may not be suitable for treating PAP in LPI patients. We suggest that alternative approaches (e.g., statins) need to be considered for clearing dying cells and cholesterol from the airways of these patients.
pulmonary alveolar proteinosis
lysinuric protein intolerance
surfactant protein D
granulocyte/monocyte colony stimulating factor
bronchoalveolar lavage fluid
whole lung lavage.
Informed consent was obtained from the parents, and the study was approved by the research ethics board at The Hospital for Sick Children. We thank Drs. Felix Ratjen and Mustafa Osman for the help in obtaining BALF samples. This project was funded by SickKids Foundation (NP) and CIHR 172874-RS (NP). D.N.D is a recipient of SickKids' Restracomp/OSOTF scholarship.
- Trapnell BC, Whitsett JA: Gm-CSF regulates pulmonary surfactant homeostasis and alveolar macrophage-mediated innate host defense. Annu Rev Physiol. 2002, 64: 775-802. 10.1146/annurev.physiol.64.090601.113847.View ArticlePubMedGoogle Scholar
- Hallman M, Merritt TA: Lack of GM-CSF as a cause of pulmonary alveolar proteinosis. J Clin Invest. 1996, 97: 589-590. 10.1172/JCI118453.PubMed CentralView ArticlePubMedGoogle Scholar
- Dirksen U, Nishinakamura R, Groneck P, Hattenhorst U, Nogee L, Murray R, Burdach S: Human pulmonary alveolar proteinosis associated with a defect in GM-CSF/IL-3/IL-5 receptor common beta chain expression. J Clin Invest. 1997, 100: 2211-2217. 10.1172/JCI119758.PubMed CentralView ArticlePubMedGoogle Scholar
- Patiroglu T, Akyildiz B, Patiroglu TE, Gulmez IY: Recurrent pulmonary alveolar proteinosis secondary to agammaglobulinemia. Pediatr Pulmonol. 2008, 43: 710-713. 10.1002/ppul.20818.View ArticlePubMedGoogle Scholar
- Inaba H, Jenkins JJ, McCarville MB, Morrison RR, Howard SC, Pui CH, Ribeiro RC: Pulmonary alveolar proteinosis in pediatric leukemia. Pediatr Blood Cancer. 2008, 51: 66-70. 10.1002/pbc.21442.View ArticlePubMedGoogle Scholar
- Sergeeva A, Ono Y, Rios R, Molldrem JJ: High titer autoantibodies to GM-CSF in patients with AML, CML and MDS are associated with active disease. Leukemia. 2008, 22: 783-790. 10.1038/sj.leu.2405104.PubMed CentralView ArticlePubMedGoogle Scholar
- Tran Van Nhieu J, Vojtek AM, Bernaudin JF, Escudier E, Fleury-Feith J: Pulmonary alveolar proteinosis associated with Pneumocystis carinii. Ultrastructural identification in bronchoalveolar lavage in AIDS and immunocompromised non-AIDS patients. Chest. 1990, 98: 801-805. 10.1378/chest.98.4.801.View ArticlePubMedGoogle Scholar
- Yamamoto H, Yamaguchi E, Agata H, Kandatsu N, Komatsu T, Kawai S, Baba K, Awaya T, Nishikomori R, Tsurusawa M, Nakata K: A combination therapy of whole lung lavage and GM-CSF inhalation in pulmonary alveolar proteinosis. Pediatr Pulmonol. 2008, 43: 828-830. 10.1002/ppul.20856.View ArticlePubMedGoogle Scholar
- Kavuru MS, Popovich M: Therapeutic whole lung lavage: a stop-gap therapy for alveolar proteinosis. Chest. 2002, 122: 1123-1124. 10.1378/chest.122.4.1123.View ArticlePubMedGoogle Scholar
- Froudarakis ME, Koutsopoulos A, Mihailidou HP: Total lung lavage by awake flexible fiberoptic bronchoscope in a 13-year-old girl with pulmonary alveolar proteinosis. Respir Med. 2007, 101: 366-369. 10.1016/j.rmed.2006.04.020.View ArticlePubMedGoogle Scholar
- Numata A, Matsuishi E, Koyanagi K, Saito S, Miyamoto Y, Irie K, Gondo H, Harada M: Successful therapy with whole-lung lavage and autologous peripheral blood stem cell transplantation for pulmonary alveolar proteinosis complicating acute myelogenous leukemia. Am J Hematol. 2006, 81: 107-109. 10.1002/ajh.20473.View ArticlePubMedGoogle Scholar
- Paschen C, Reiter K, Stanzel F, Teschler H, Griese M: Therapeutic lung lavages in children and adults. Respir Res. 2005, 6: 138-10.1186/1465-9921-6-138.PubMed CentralView ArticlePubMedGoogle Scholar
- Tazawa R, Hamano E, Arai T, Ohta H, Ishimoto O, Uchida K, Watanabe M, Saito J, Takeshita M, Hirabayashi Y, Ishige I, Eishi Y, Hagiwara K, Ebina M, Inoue Y, Nakata K, Nukiwa T: Granulocyte-macrophage colony-stimulating factor and lung immunity in pulmonary alveolar proteinosis. Am J Respir Crit Care Med. 2005, 171: 1142-1149. 10.1164/rccm.200406-716OC.View ArticlePubMedGoogle Scholar
- Kavuru MS, Sullivan EJ, Piccin R, Thomassen MJ, Stoller JK: Exogenous granulocyte-macrophage colony-stimulating factor administration for pulmonary alveolar proteinosis. Am J Respir Crit Care Med. 2000, 161: 1143-1148.View ArticlePubMedGoogle Scholar
- Price A, Manson D, Cutz E, Dell S: Pulmonary alveolar proteinosis associated with anti-GM-CSF antibodies in a child: successful treatment with inhaled GM-CSF. Pediatr Pulmonol. 2006, 41: 367-370. 10.1002/ppul.20347.View ArticlePubMedGoogle Scholar
- Seymour JF, Presneill JJ, Schoch OD, Downie GH, Moore PE, Doyle IR, Vincent JM, Nakata K, Kitamura T, Langton D, Pain MC, Dunn AR: Therapeutic efficacy of granulocyte-macrophage colony-stimulating factor in patients with idiopathic acquired alveolar proteinosis. Am J Respir Crit Care Med. 2001, 163: 524-531.View ArticlePubMedGoogle Scholar
- Akagawa KS, Kamoshita K, Tokunaga T: Effects of granulocyte-macrophage colony-stimulating factor and colony-stimulating factor-1 on the proliferation and differentiation of murine alveolar macrophages. J Immunol. 1988, 141: 3383-3390.PubMedGoogle Scholar
- Just U, Stocking C, Spooncer E, Dexter TM, Ostertag W: Expression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation. Cell. 1991, 64: 1163-1173. 10.1016/0092-8674(91)90271-Y.View ArticlePubMedGoogle Scholar
- Shibata Y, Berclaz PY, Chroneos ZC, Yoshida M, Whitsett JA, Trapnell BC: GM-CSF regulates alveolar macrophage differentiation and innate immunity in the lung through PU.1. Immunity. 2001, 15: 557-567. 10.1016/S1074-7613(01)00218-7.View ArticlePubMedGoogle Scholar
- Lang RA, Metcalf D, Cuthbertson RA, Lyons I, Stanley E, Kelso A, Kannourakis G, Williamson DJ, Klintworth GK, Gonda TJ, Dunn AR: Transgenic mice expressing a hemopoietic growth factor gene (GM-CSF) develop accumulations of macrophages, blindness, and a fatal syndrome of tissue damage. Cell. 1987, 51: 675-686. 10.1016/0092-8674(87)90136-X.View ArticlePubMedGoogle Scholar
- Ikegami M, Hull WM, Yoshida M, Wert SE, Whitsett JA: SP-D and GM-CSF regulate surfactant homeostasis via distinct mechanisms. Am J Physiol Lung Cell Mol Physiol. 2001, 281: L697-703.PubMedGoogle Scholar
- Ikegami M, Ueda T, Hull W, Whitsett JA, Mulligan RC, Dranoff G, Jobe AH: Surfactant metabolism in transgenic mice after granulocyte macrophage-colony stimulating factor ablation. Am J Physiol. 1996, 270: L650-658.PubMedGoogle Scholar
- Uchida K, Beck DC, Yamamoto T, Berclaz PY, Abe S, Staudt MK, Carey BC, Filippi MD, Wert SE, Denson LA, Puchalski JT, Hauck DM, Trapnell BC: GM-CSF autoantibodies and neutrophil dysfunction in pulmonary alveolar proteinosis. N Engl J Med. 2007, 356: 567-579. 10.1056/NEJMoa062505.View ArticlePubMedGoogle Scholar
- Ikegami M, Whitsett JA, Jobe A, Ross G, Fisher J, Korfhagen T: Surfactant metabolism in SP-D gene-targeted mice. Am J Physiol Lung Cell Mol Physiol. 2000, 279: L468-476.PubMedGoogle Scholar
- Korfhagen TR, Sheftelyevich V, Burhans MS, Bruno MD, Ross GF, Wert SE, Stahlman MT, Jobe AH, Ikegami M, Whitsett JA, Fisher JH: Surfactant protein-D regulates surfactant phospholipid homeostasis in vivo. J Biol Chem. 1998, 273: 28438-28443. 10.1074/jbc.273.43.28438.View ArticlePubMedGoogle Scholar
- Botas C, Poulain F, Akiyama J, Brown C, Allen L, Goerke J, Clements J, Carlson E, Gillespie AM, Epstein C, Hawgood S: Altered surfactant homeostasis and alveolar type II cell morphology in mice lacking surfactant protein D. Proc Natl Acad Sci USA. 1998, 95: 11869-11874. 10.1073/pnas.95.20.11869.PubMed CentralView ArticlePubMedGoogle Scholar
- Palaniyar N, Sorensen G, Holmskov U: Immunoregulatory Roles of Lung Surfactant Protein A and D. Animal Lectin: A functional view. Edited by: Vasta G, Ahmed I. 2008, CRC Press, 331-342.Google Scholar
- Palaniyar N, Clark H, Nadesalingam J, Shih MJ, Hawgood S, Reid KB: Innate immune collectin surfactant protein D enhances the clearance of DNA by macrophages and minimizes anti-DNA antibody generation. J Immunol. 2005, 174: 7352-7358.View ArticlePubMedGoogle Scholar
- Giannoni E, Sawa T, Allen L, Wiener-Kronish J, Hawgood S: Surfactant proteins A and D enhance pulmonary clearance of Pseudomonas aeruginosa. Am J Respir Cell Mol Biol. 2006, 34: 704-710. 10.1165/rcmb.2005-0461OC.PubMed CentralView ArticlePubMedGoogle Scholar
- Clark H, Palaniyar N, Strong P, Edmondson J, Hawgood S, Reid KB: Surfactant protein D reduces alveolar macrophage apoptosis in vivo. J Immunol. 2002, 169: 2892-2899.View ArticlePubMedGoogle Scholar
- Fisher JH, Sheftelyevich V, Ho YS, Fligiel S, McCormack FX, Korfhagen TR, Whitsett JA, Ikegami M: Pulmonary-specific expression of SP-D corrects pulmonary lipid accumulation in SP-D gene-targeted mice. Am J Physiol Lung Cell Mol Physiol. 2000, 278: L365-373.PubMedGoogle Scholar
- Parto K, Svedstrom E, Majurin ML, Harkonen R, Simell O: Pulmonary manifestations in lysinuric protein intolerance. Chest. 1993, 104: 1176-1182. 10.1378/chest.104.4.1176.View ArticlePubMedGoogle Scholar
- Parto K, Kallajoki M, Aho H, Simell O: Pulmonary alveolar proteinosis and glomerulonephritis in lysinuric protein intolerance: case reports and autopsy findings of four pediatric patients. Hum Pathol. 1994, 25: 400-407. 10.1016/0046-8177(94)90150-3.View ArticlePubMedGoogle Scholar
- Santamaria F, Brancaccio G, Parenti G, Francalanci P, Squitieri C, Sebastio G, Dionisi-Vici C, D'Argenio P, Andria G, Parisi F: Recurrent fatal pulmonary alveolar proteinosis after heart-lung transplantation in a child with lysinuric protein intolerance. J Pediatr. 2004, 145: 268-272. 10.1016/j.jpeds.2004.04.047.View ArticlePubMedGoogle Scholar
- Torrents D, Mykkanen J, Pineda M, Feliubadalo L, Estevez R, de Cid R, Sanjurjo P, Zorzano A, Nunes V, Huoponen K, Reinikainen A, Simell O, Savontaus ML, Aula P, Palacin M: Identification of SLC7A7, encoding y+LAT-1, as the lysinuric protein intolerance gene. Nat Genet. 1999, 21: 293-296. 10.1038/6809.View ArticlePubMedGoogle Scholar
- Borsani G, Bassi MT, Sperandeo MP, De Grandi A, Buoninconti A, Riboni M, Manzoni M, Incerti B, Pepe A, Andria G, Ballabio A, Sebastio G: SLC7A7, encoding a putative permease-related protein, is mutated in patients with lysinuric protein intolerance. Nat Genet. 1999, 21: 297-301. 10.1038/6815.View ArticlePubMedGoogle Scholar
- Broer S: Lysinuric protein intolerance: one gene, many problems. Am J Physiol Cell Physiol. 2007, 293: C540-541. 10.1152/ajpcell.00166.2007.View ArticlePubMedGoogle Scholar
- Tanner LM, Nanto-Salonen K, Rashed MS, Kotilainen S, Aalto M, Venetoklis J, Niinikoski H, Huoponen K, Simell O: Carnitine deficiency and L-carnitine supplementation in lysinuric protein intolerance. 0026-0495. 2008, 57: 549-554.PubMedGoogle Scholar
- Kerem E, Elpelg ON, Shalev RS, Rosenman E, Bar Ziv Y, Branski D: Lysinuric protein intolerance with chronic interstitial lung disease and pulmonary cholesterol granulomas at onset. J Pediatr. 1993, 123: 275-278. 10.1016/S0022-3476(05)81703-2.View ArticlePubMedGoogle Scholar
- Nadesalingam J, Bernal AL, Dodds AW, Willis AC, Mahoney DJ, Day AJ, Reid KB, Palaniyar N: Identification and characterization of a novel interaction between pulmonary surfactant protein D and decorin. J Biol Chem. 2003, 278: 25678-25687. 10.1074/jbc.M210186200.View ArticlePubMedGoogle Scholar
- Palaniyar N, Nadesalingam J, Clark H, Shih MJ, Dodds AW, Reid KB: Nucleic acid is a novel ligand for innate, immune pattern recognition collectins surfactant proteins A and D and mannose-binding lectin. J Biol Chem. 2004, 279: 32728-32736. 10.1074/jbc.M403763200.View ArticlePubMedGoogle Scholar
- Palaniyar N, Zhang L, Kuzmenko A, Ikegami M, Wan S, Wu H, Korfhagen TR, Whitsett JA, McCormack FX: The role of pulmonary collectin N-terminal domains in surfactant structure, function, and homeostasis in vivo. J Biol Chem. 2002, 277: 26971-26979. 10.1074/jbc.M110080200.View ArticlePubMedGoogle Scholar
- Poulain FR, Akiyama J, Allen L, Brown C, Chang R, Goerke J, Dobbs L, Hawgood S: Ultrastructure of phospholipid mixtures reconstituted with surfactant proteins B and D. Am J Respir Cell Mol Biol. 1999, 20: 1049-1058.View ArticlePubMedGoogle Scholar
- Kinchen JM, Ravichandran KS: Phagosome maturation: going through the acid test. Nat Rev Mol Cell Biol. 2008, 9: 781-795. 10.1038/nrm2515.PubMed CentralView ArticlePubMedGoogle Scholar
- Sperandeo MP, Bassi MT, Riboni M, Parenti G, Buoninconti A, Manzoni M, Incerti B, Larocca MR, Di Rocco M, Strisciuglio P, Dianzani I, Parini R, Candito M, Endo F, Ballabio A, Andria G, Sebastio G, Borsani G: Structure of the SLC7A7 gene and mutational analysis of patients affected by lysinuric protein intolerance. Am J Hum Genet. 2000, 66: 92-99. 10.1086/302700.PubMed CentralView ArticlePubMedGoogle Scholar
- Ceruti M, Rodi G, Stella GM, Adami A, Bolongaro A, Baritussio A, Pozzi E, Luisetti M: Successful whole lung lavage in pulmonary alveolar proteinosis secondary to lysinuric protein intolerance: a case report. Orphanet J Rare Dis. 2007, 2: 14-10.1186/1750-1172-2-14.PubMed CentralView ArticlePubMedGoogle Scholar
- Sperandeo MP, Annunziata P, Ammendola V, Fiorito V, Pepe A, Soldovieri MV, Taglialatela M, Andria G, Sebastio G: Lysinuric protein intolerance: identification and functional analysis of mutations of the SLC7A7 gene. Hum Mutat. 2005, 25: 410-10.1002/humu.9323.View ArticlePubMedGoogle Scholar
- Koizumi A, Shoji Y, Nozaki J, Noguchi A, E X, Dakeishi M, Ohura T, Tsuyoshi K, Yasuhiko W, Manabe M, Takasago Y, Takada G: A cluster of lysinuric protein intolerance (LPI) patients in a northern part of Iwate, Japan due to a founder effect. The Mass Screening Group. Hum Mutat. 2000, 16: 270-271. 10.1002/1098-1004(200009)16:3<270::AID-HUMU14>3.0.CO;2-J.View ArticlePubMedGoogle Scholar
- Sperandeo MP, Andria G, Sebastio G: Lysinuric protein intolerance: update and extended mutation analysis of the SLC7A7 gene. Hum Mutat. 2008, 29: 14-21. 10.1002/humu.20589.View ArticlePubMedGoogle Scholar
- Alcorn JF, Wright JR: Degradation of pulmonary surfactant protein D by Pseudomonas aeruginosa elastase abrogates innate immune function. J Biol Chem. 2004, 279: 30871-30879. 10.1074/jbc.M400796200.View ArticlePubMedGoogle Scholar
- Hirche TO, Crouch EC, Espinola M, Brokelman TJ, Mecham RP, DeSilva N, Cooley J, Remold-O'Donnell E, Belaaouaj A: Neutrophil serine proteinases inactivate surfactant protein D by cleaving within a conserved subregion of the carbohydrate recognition domain. J Biol Chem. 2004, 279: 27688-27698. 10.1074/jbc.M402936200.View ArticlePubMedGoogle Scholar
- Palaniyar N, Ikegami M, Korfhagen T, Whitsett J, McCormack FX: Domains of surfactant protein A that affect protein oligomerization, lipid structure and surface tension. Comp Biochem Physiol A Mol Integr Physiol. 2001, 129: 109-127. 10.1016/S1095-6433(01)00309-9.View ArticlePubMedGoogle Scholar
- Meaney S, Bonfield TL, Hansson M, Babiker A, Kavuru MS, Thomassen MJ: Serum cholestenoic acid as a potential marker of pulmonary cholesterol homeostasis: increased levels in patients with pulmonary alveolar proteinosis. J Lipid Res. 2004, 45: 2354-2360. 10.1194/jlr.M400302-JLR200.View ArticlePubMedGoogle Scholar
- Keating E, Rahman L, Francis J, Petersen A, Possmayer F, Veldhuizen R, Petersen NO: Effect of cholesterol on the biophysical and physiological properties of a clinical pulmonary surfactant. Biophys J. 2007, 93: 1391-1401. 10.1529/biophysj.106.099762.PubMed CentralView ArticlePubMedGoogle Scholar
- Leonenko Z, Gill S, Baoukina S, Monticelli L, Doehner J, Gunasekara L, Felderer F, Rodenstein M, Eng LM, Amrein M: An elevated level of cholesterol impairs self-assembly of pulmonary surfactant into a functional film. Biophys J. 2007, 93: 674-683. 10.1529/biophysj.107.106310.PubMed CentralView ArticlePubMedGoogle Scholar
- Morimoto K, Janssen WJ, Fessler MB, McPhillips KA, Borges VM, Bowler RP, Xiao YQ, Kench JA, Henson PM, Vandivier RW: Lovastatin enhances clearance of apoptotic cells (efferocytosis) with implications for chronic obstructive pulmonary disease. J Immunol. 2006, 176: 7657-7665.View ArticlePubMedGoogle Scholar
- Kitamura T, Tanaka N, Watanabe J, Uchida , Kanegasaki S, Yamada Y, Nakata K: Idiopathic pulmonary alveolar proteinosis as an autoimmune disease with neutralizing antibody against granulocyte/macrophage colony-stimulating factor. J Exp Med. 1999, 190: 875-880. 10.1084/jem.190.6.875.PubMed CentralView ArticlePubMedGoogle Scholar
- Dranoff G, Crawford AD, Sadelain M, Ream B, Rashid A, Bronson RT, Dickersin GR, Bachurski CJ, Mark EL, Whitsett JA, Mulligan RC: Involvement of granulocyte-macrophage colony-stimulating factor in pulmonary homeostasis. Science. 1994, 264: 713-716. 10.1126/science.8171324.View ArticlePubMedGoogle Scholar
- Huffman JA, Hull WM, Dranoff G, Mulligan RC, Whitsett JA: Pulmonary epithelial cell expression of GM-CSF corrects the alveolar proteinosis in GM-CSF-deficient mice. J Clin Invest. 1996, 97: 649-655. 10.1172/JCI118461.PubMed CentralView ArticlePubMedGoogle Scholar
- Martinez-Moczygemba M, Doan ML, Elidemir O, Fan LL, Cheung SW, Lei JT, Moore JP, Tavana G, Lewis LR, Zhu Y, Muzny DM, Gibbs RA, Huston DP: Pulmonary alveolar proteinosis caused by deletion of the GM-CSFRalpha gene in the X chromosome pseudoautosomal region 1. J Exp Med. 2008, 205: 2711-2716. 10.1084/jem.20080759.PubMed CentralView ArticlePubMedGoogle Scholar
- Suzuki T, Sakagami T, Rubin BK, Nogee LM, Wood RE, Zimmerman SL, Smolarek T, Dishop MK, Wert SE, Whitsett JA, Grabowski G, Carey BC, Stevens C, Loo van der JC, Trapnell BC: Familial pulmonary alveolar proteinosis caused by mutations in CSF2RA. J Exp Med. 2008, 205: 2703-2710. 10.1084/jem.20080990.PubMed CentralView ArticlePubMedGoogle Scholar
- Nishinakamura R, Wiler R, Dirksen U, Morikawa Y, Arai K, Miyajima A, Burdach S, Murray R: The pulmonary alveolar proteinosis in granulocyte macrophage colony-stimulating factor/interleukins 3/5 beta c receptor-deficient mice is reversed by bone marrow transplantation. J Exp Med. 1996, 183: 2657-2662. 10.1084/jem.183.6.2657.View ArticlePubMedGoogle Scholar
- Yoshida M, Ikegami M, Reed JA, Chroneos ZC, Whitsett JA: GM-CSF regulates protein and lipid catabolism by alveolar macrophages. Am J Physiol Lung Cell Mol Physiol. 2001, 280: L379-386.PubMedGoogle Scholar
- Vandivier RW, Ogden CA, Fadok VA, Hoffmann PR, Brown KK, Botto M, Walport MJ, Fisher JH, Henson PM, Greene KE: Role of surfactant proteins A, D, and C1q in the clearance of apoptotic cells in vivo and in vitro: calreticulin and CD91 as a common collectin receptor complex. J Immunol. 2002, 169: 3978-3986.View ArticlePubMedGoogle Scholar
- Koth LL, Alex B, Hawgood S, Nead MA, Sheppard D, Erle DJ, Morris DG: Integrin beta6 mediates phospholipid and collectin homeostasis by activation of latent TGF-beta1. Am J Respir Cell Mol Biol. 2007, 37: 651-659. 10.1165/rcmb.2006-0428OC.PubMed CentralView ArticlePubMedGoogle Scholar
- Doan ML, Guillerman RP, Dishop MK, Nogee LM, Langston C, Mallory GB, Sockrider MM, Fan LL: Clinical, radiological and pathological features of ABCA3 mutations in children. Thorax. 2008, 63: 366-373. 10.1136/thx.2007.083766.View ArticlePubMedGoogle Scholar
- Thomassen MJ, Barna BP, Malur AG, Bonfield TL, Farver CF, Malur A, Dalrymple H, Kavuru MS, Febbraio M: ABCG1 is deficient in alveolar macrophages of GM-CSF knockout mice and patients with pulmonary alveolar proteinosis. J Lipid Res. 2007, 48: 2762-2768. 10.1194/jlr.P700022-JLR200.View ArticlePubMedGoogle Scholar
- Baldan A, Tarr P, Vales CS, Frank J, Shimotake TK, Hawgood S, Edwards PA: Deletion of the transmembrane transporter ABCG1 results in progressive pulmonary lipidosis. J Biol Chem. 2006, 281: 29401-29410. 10.1074/jbc.M606597200.View ArticlePubMedGoogle Scholar
- Elliott MJ, Strasser A, Metcalf D: Selective up-regulation of macrophage function in granulocyte-macrophage colony-stimulating factor transgenic mice. J Immunol. 1991, 147: 2957-2963.PubMedGoogle Scholar
- Wylam ME, Ten R, Prakash UB, Nadrous HF, Clawson ML, Anderson PM: Aerosol granulocyte-macrophage colony-stimulating factor for pulmonary alveolar proteinosis. Eur Respir J. 2006, 27: 585-593. 10.1183/09031936.06.00058305.View ArticlePubMedGoogle Scholar
- Arai T, Hamano E, Inoue Y, Ryushi T, Nukiwa T, Sakatani M, Nakata K: Serum neutralizing capacity of GM-CSF reflects disease severity in a patient with pulmonary alveolar proteinosis successfully treated with inhaled GM-CSF. Respir Med. 2004, 98: 1227-1230. 10.1016/j.rmed.2004.08.011.View ArticlePubMedGoogle Scholar
- Schoch OD, Schanz U, Koller M, Nakata K, Seymour JF, Russi EW, Boehler A: BAL findings in a patient with pulmonary alveolar proteinosis successfully treated with GM-CSF. Thorax. 2002, 57: 277-280. 10.1136/thorax.57.3.277.PubMed CentralView ArticlePubMedGoogle Scholar
- Hawgood S, Akiyama J, Brown C, Allen L, Li G, Poulain FR: GM-CSF mediates alveolar macrophage proliferation and type II cell hypertrophy in SP-D gene-targeted mice. Am J Physiol Lung Cell Mol Physiol. 2001, 280: L1148-1156.PubMedGoogle Scholar
- Xing Z, Tremblay GM, Sime PJ, Gauldie J: Overexpression of granulocyte-macrophage colony-stimulating factor induces pulmonary granulation tissue formation and fibrosis by induction of transforming growth factor-beta 1 and myofibroblast accumulation. Am J Pathol. 1997, 150: 59-66.PubMed CentralPubMedGoogle Scholar
- Xing Z, Ohkawara Y, Jordana M, Graham F, Gauldie J: Transfer of granulocyte-macrophage colony-stimulating factor gene to rat lung induces eosinophilia, monocytosis, and fibrotic reactions. J Clin Invest. 1996, 97: 1102-1110. 10.1172/JCI118503.PubMed CentralView ArticlePubMedGoogle Scholar
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